Syzygium malaccense leaves methanol extract modulate some biochemical and inflammatory markers and prostate histology of testosterone-estradiol valerate induced benign prostatic hyperplasia in rats.
{"title":"<i>Syzygium malaccense</i> leaves methanol extract modulate some biochemical and inflammatory markers and prostate histology of testosterone-estradiol valerate induced benign prostatic hyperplasia in rats.","authors":"Ngozi Kalu Achi, Chinedum Ogbonnaya Eleazu, Chimaraoke Onyeabo, Winner Kalu, Kate Eleazu","doi":"10.22038/AJP.2023.23526","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>The effect of <i>Syzygium malaccense</i> methanol leaf extract (SMLE) on some parameters of testosterone-estradiol valerate induced benign prostatic hyperplasia (BPH) in rats was assayed.</p><p><strong>Materials and methods: </strong>Thirty male albino rats were used and they were grouped as: Control: received 1 mL/kg olive oil (oral and subcutaneous); BPH: received subcutaneously 9 mg/kg dihydrotestosterone (DHT)+0.9 mg/kg estradiol valerate (ESV) and orally 1 ml/kg olive oil; finasteride: received 9 mg/kg of DHT+0.9 mg/kg ESV (subcutaneously) and 5 mg/kg finasteride (orally) and test groups 1 and 2: received 9 mg/kg of DHT+0.9 mg/kg ESV (subcutaneously) and 200 and 400 mg/kg SMLE (orally). The duration of the treatment was 28 days.</p><p><strong>Results: </strong>The BPH group had increased prostatic total proteins, oxidative stress, interleukin 8, tumor necrosis factor-α, prostate weights, serum concentrations of prostate specific antigen, estradiol, follicle stimulating hormone, and C-reactive protein, dyslipidaemia, altered prostate histology and hormonal levels but had no significant change (p>0.05) in haematological indices relative to the control. Finasteride or <i>S</i>. <i>malaccense</i> modulated most of these parameters as corroborated by prostate histology. Acute toxicity study indicated the non-toxicity of SMLE. SMLE showed strong <i>in vitro</i> antioxidant activity which corroborated its <i>in vivo</i> antioxidant activity.</p><p><strong>Conclusion: </strong>The study showed that <i>S</i>. <i>malaccense</i> could be useful in the management of BPH.</p>","PeriodicalId":8677,"journal":{"name":"Avicenna Journal of Phytomedicine","volume":"14 3","pages":"305-324"},"PeriodicalIF":1.9000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11287027/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Avicenna Journal of Phytomedicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22038/AJP.2023.23526","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: The effect of Syzygium malaccense methanol leaf extract (SMLE) on some parameters of testosterone-estradiol valerate induced benign prostatic hyperplasia (BPH) in rats was assayed.
Materials and methods: Thirty male albino rats were used and they were grouped as: Control: received 1 mL/kg olive oil (oral and subcutaneous); BPH: received subcutaneously 9 mg/kg dihydrotestosterone (DHT)+0.9 mg/kg estradiol valerate (ESV) and orally 1 ml/kg olive oil; finasteride: received 9 mg/kg of DHT+0.9 mg/kg ESV (subcutaneously) and 5 mg/kg finasteride (orally) and test groups 1 and 2: received 9 mg/kg of DHT+0.9 mg/kg ESV (subcutaneously) and 200 and 400 mg/kg SMLE (orally). The duration of the treatment was 28 days.
Results: The BPH group had increased prostatic total proteins, oxidative stress, interleukin 8, tumor necrosis factor-α, prostate weights, serum concentrations of prostate specific antigen, estradiol, follicle stimulating hormone, and C-reactive protein, dyslipidaemia, altered prostate histology and hormonal levels but had no significant change (p>0.05) in haematological indices relative to the control. Finasteride or S. malaccense modulated most of these parameters as corroborated by prostate histology. Acute toxicity study indicated the non-toxicity of SMLE. SMLE showed strong in vitro antioxidant activity which corroborated its in vivo antioxidant activity.
Conclusion: The study showed that S. malaccense could be useful in the management of BPH.