Molecular Study of Viral Causes of Childhood Diarrhea in Western Maharashtra

Q4 Medicine
Mahima Lall, R. M. Reddy, Yasmin Muhammed, Sourav Sen, R. Gupta
{"title":"Molecular Study of Viral Causes of Childhood Diarrhea in Western Maharashtra","authors":"Mahima Lall, R. M. Reddy, Yasmin Muhammed, Sourav Sen, R. Gupta","doi":"10.4103/mjdrdypu.mjdrdypu_846_23","DOIUrl":null,"url":null,"abstract":"ABSTRACT\n \n \n \n Although diarrheal diseases are highly preventable, they remain one of the leading causes of mortality and morbidity in the pediatric population in developing countries. Knowledge of etiological agents is essential to implement public health programs. Viral infections are the most common cause of acute infectious diarrhea among children. There is a paucity of information regarding viral enteropathogens responsible for diarrhea in India, as many hospitals do not routinely carry out diagnostic tests for viral detection.\n \n \n \n To estimate the prevalence of viral enteropathogens in children under five years of age presenting with acute diarrhea using real-time multiplex polymerase chain reaction (PCR) and its correlation with clinico-demographic features.\n \n \n \n A cross-sectional study was carried out in a tertiary care center in Maharashtra from January 2016 to June 2019. Our study included 300 children who were under five years old with acute diarrhea. Standard protocols were advocated for the collection and transportation of stool samples. All samples were subjected to real-time multiplex PCR (Light cycler 480 Roche) using Fast Track Diagnostic kits for viral gastroenteritis. Data analysis was done using descriptive and analytical methods.\n \n \n \n In our study, 57% of samples were positive for viral enteropathogens. Rotavirus (32%) was the most common isolate, followed by Norovirus Genogroup II (GII) (17%). The majority of pathogens were isolated from children less than 2 years of age.\n \n \n \n There is a need to test stool specimens of clinically confirmed diarrheal patients for enteric virus. This will avoid blind antibiotic treatment.\n","PeriodicalId":18412,"journal":{"name":"Medical Journal of Dr. D.Y. Patil Vidyapeeth","volume":"2000 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medical Journal of Dr. D.Y. Patil Vidyapeeth","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/mjdrdypu.mjdrdypu_846_23","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0

Abstract

ABSTRACT Although diarrheal diseases are highly preventable, they remain one of the leading causes of mortality and morbidity in the pediatric population in developing countries. Knowledge of etiological agents is essential to implement public health programs. Viral infections are the most common cause of acute infectious diarrhea among children. There is a paucity of information regarding viral enteropathogens responsible for diarrhea in India, as many hospitals do not routinely carry out diagnostic tests for viral detection. To estimate the prevalence of viral enteropathogens in children under five years of age presenting with acute diarrhea using real-time multiplex polymerase chain reaction (PCR) and its correlation with clinico-demographic features. A cross-sectional study was carried out in a tertiary care center in Maharashtra from January 2016 to June 2019. Our study included 300 children who were under five years old with acute diarrhea. Standard protocols were advocated for the collection and transportation of stool samples. All samples were subjected to real-time multiplex PCR (Light cycler 480 Roche) using Fast Track Diagnostic kits for viral gastroenteritis. Data analysis was done using descriptive and analytical methods. In our study, 57% of samples were positive for viral enteropathogens. Rotavirus (32%) was the most common isolate, followed by Norovirus Genogroup II (GII) (17%). The majority of pathogens were isolated from children less than 2 years of age. There is a need to test stool specimens of clinically confirmed diarrheal patients for enteric virus. This will avoid blind antibiotic treatment.
马哈拉施特拉邦西部儿童腹泻病毒病因的分子研究
摘要 尽管腹泻疾病极易预防,但它仍然是发展中国家儿童死亡和发病的主要原因之一。了解病原体对于实施公共卫生计划至关重要。病毒感染是儿童急性感染性腹泻最常见的病因。在印度,由于许多医院没有对病毒检测进行常规诊断测试,因此有关导致腹泻的病毒性肠道病原体的信息非常缺乏。 利用实时多重聚合酶链反应(PCR)估算五岁以下急性腹泻患儿中病毒性肠道病原体的流行率及其与临床人口学特征的相关性。 2016年1月至2019年6月,我们在马哈拉施特拉邦的一家三级医疗中心开展了一项横断面研究。我们的研究包括 300 名五岁以下患有急性腹泻的儿童。粪便样本的采集和运输均采用标准方案。所有样本均使用病毒性肠胃炎快速诊断试剂盒进行实时多重 PCR(Light cycler 480 Roche)检测。数据分析采用描述性和分析性方法。 在我们的研究中,57%的样本对病毒性肠道病原体呈阳性反应。轮状病毒(32%)是最常见的分离物,其次是诺罗病毒基因 II 组(GII)(17%)。大多数病原体是从 2 岁以下儿童中分离出来的。 有必要对临床确诊的腹泻患者的粪便标本进行肠道病毒检测。这将避免盲目使用抗生素治疗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
0.30
自引率
0.00%
发文量
221
审稿时长
43 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信