{"title":"Automated image processing algorithm for 3D OCT images of fouling in spacer-filled membrane filtration channels","authors":"","doi":"10.1016/j.mex.2024.102871","DOIUrl":null,"url":null,"abstract":"<div><p>OCT imaging is an important technique to study fouling in spacer-filled channels of reverse osmosis systems for seawater desalination. However, OCT imaging of membrane filtration channels with feed spacers is challenging because the spacer material can be (partly) transparent, making it difficult to detect and possibly mistaken for fouling, and the longer optical pathway through the spacer material distorts the image below the spacer. This study presents an automated 3D OCT image processing method in MATLAB for visualization and quantification of biofouling in spacer-filled channels. First, a spacer template of arbitrary size and rotation was generated from a CT scan of the feed spacer. Second, background noise and file size were reduced by representing the OCT image with a list of discrete reflectors. Finally, the spacer template was overlayed with the feed spacer in the 3D OCT image, enabling automated visualization of the feed spacer and correction of the distortions. Moreover, the method allows the selection of datasets with the same location relative to the position of the spacer, enabling systematic comparison between datasets and quantitative analysis.</p><ul><li><span>•</span><span><p>A spacer template of arbitrary size and rotation was generated from a CT scan.</p></span></li><li><span>•</span><span><p>The background noise was removed, and the file size was reduced by representing the OCT dataset with a list of discrete reflectors.</p></span></li><li><span>•</span><span><p>The spacer template was overlayed with the feed spacer in the 3D OCT image.</p></span></li></ul></div>","PeriodicalId":18446,"journal":{"name":"MethodsX","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2215016124003236/pdfft?md5=012a0b41809fc0675396e93d62ecd1c4&pid=1-s2.0-S2215016124003236-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"MethodsX","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2215016124003236","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
OCT imaging is an important technique to study fouling in spacer-filled channels of reverse osmosis systems for seawater desalination. However, OCT imaging of membrane filtration channels with feed spacers is challenging because the spacer material can be (partly) transparent, making it difficult to detect and possibly mistaken for fouling, and the longer optical pathway through the spacer material distorts the image below the spacer. This study presents an automated 3D OCT image processing method in MATLAB for visualization and quantification of biofouling in spacer-filled channels. First, a spacer template of arbitrary size and rotation was generated from a CT scan of the feed spacer. Second, background noise and file size were reduced by representing the OCT image with a list of discrete reflectors. Finally, the spacer template was overlayed with the feed spacer in the 3D OCT image, enabling automated visualization of the feed spacer and correction of the distortions. Moreover, the method allows the selection of datasets with the same location relative to the position of the spacer, enabling systematic comparison between datasets and quantitative analysis.
•
A spacer template of arbitrary size and rotation was generated from a CT scan.
•
The background noise was removed, and the file size was reduced by representing the OCT dataset with a list of discrete reflectors.
•
The spacer template was overlayed with the feed spacer in the 3D OCT image.
OCT 成像是研究海水淡化反渗透系统中充满间隔物的通道污垢的重要技术。然而,对带有进料隔层的膜过滤通道进行 OCT 成像具有挑战性,因为隔层材料可能是(部分)透明的,使其难以检测并可能被误认为是污垢,而且穿过隔层材料的较长光路扭曲了隔层下方的图像。本研究在 MATLAB 中提出了一种自动三维 OCT 图像处理方法,用于可视化和量化充满间隔物的通道中的生物污垢。首先,根据进样间隔物的 CT 扫描结果生成任意大小和旋转的间隔物模板。其次,用离散反射体列表来表示 OCT 图像,从而减少了背景噪声和文件大小。最后,在三维 OCT 图像中将馈源间隔模板与馈源间隔叠加,从而实现了馈源间隔的自动可视化和畸变校正。此外,该方法还可以选择与间隔物位置相对应的相同位置的数据集,从而实现数据集之间的系统比较和定量分析。