Feed characteristics and potential effects on ruminal bacteria of ensiled sugar kelp and winged kelp for Holstein dairy cows

Abstract

Seaweed silage has potential as an alternative feed ingredient for dairy cows. This study aims to investigate seaweed’s and seaweed silageś nutrient digestibility as well as their impact on the ruminal bacterial composition. The cultivated S. latissima and A. esculenta were preserved by freezing at − 40 °C or ensiling (16 °C, 3 months) with four different treatments: no additives, 4 g formic acid/kg wet seaweed, lactic acid bacteria (LAB) inoculant, and LAB inoculant in prewilted biomass (ca. 300 g DM/kg wet biomass). The nutrient digestibility was estimated using standard feed evaluation methods. The bacterial composition in ruminal fluid after 48 h in vitro anaerobic incubation with seaweeds and common feedstuffs was analysed using 16S ribosomal RNA (rRNA) amplicon sequencing (V3-V4) and quantitative PCR (qPCR). The results suggest that S. latissima was more digestible than A. esculenta and that the preservation treatments had only a small effect on the nutrient digestibility and ruminal bacteria compositions. The rumen DM degradability of S. latissima was comparable to common perennial and corn forage; however, the total tract CP digestibility of S. latissima (460 g/kg CP) was lower than common forages (620 – 820 g/kg CP) and was not improved by ensiling. There was a lack of insoluble but rumen−degradable CP in A. esculenta, making it unsuitable as a nutrient ingredient for dairy cows. The ruminal bacterial composition changed depending on the seaweed species used as substrate: The dominant bacterial taxa when incubated with S. latissima belonged to the genus Prevotella (relative abundance: 79 – 93%), known for its ability to degrade polysaccharides in various ecosystems. Moreover, the fibrolytic bacteria including Fibrobacter succinogenes and Ruminococcus flavefaciens were > 2.5 Log2FoldChange higher when incubating with S. latissima than with A. esculenta. These bacterial taxa may play an important role in the in vitro organic matter digestibility, noted as 2 times higher in S. latissima compared to A. esculenta. The qPCR results indicated potential methane mitigation properties of the studied seaweed species, with significantly lower gene copies of Archaea 16S rRNA and methyl coenzyme-M reductase subunit A genes when the ruminal fluid was incubated with the seaweed substrates. Our study suggested that ensiled S. latissima biomass can be included in the diet of dairy cows as an alternative forage-like ingredient with the potential of methane mitigation.