SNPgen: A portal of innovative automated tools for genotyping assay design

Abstract

SNPgen is a web portal that simplifies primer design for the detection of single nucleotide polymorphisms (SNPs) and insertions/deletions (indels). It offers user-friendly tools for automating primer design, retrieve SNP details and generate primers for various genotyping methods such as ARMS-PCR and high-resolution melt (HRM) analysis. SNPgen considers factors such as GC content and melting temperature for optimal primers and allows visualization of amplicons and primers. This user-friendly portal can revolutionize genotyping workflows in various research areas.