Postponing first colostrum collection: impact on immunoglobulin G in goat colostrum

IF 4 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
F. Zamuner , A.W.N. Cameron , E.K. Carpenter , G. Arcos-Gómez , B.J. Leury , K. DiGiacomo
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引用次数: 0

Abstract

This experiment was motivated by the need to understand the impacts of delaying the first colostrum collection on immunoglobulin G (IgG) concentrations in goat colostrum, addressing a gap in caprine-specific research, despite its significance in dairy farming. Concurrently, we examined the relationship between colostral IgG, total protein (TP) and Brix values. Two colostrum samples were collected from 56 Saanen goats, one from each udder half. The first sample was collected from the right teat immediately postbirth, and the second sample was collected from the left teat at one of the predetermined postpartum intervals: 0, 4–6, 8–10, or 12–14 h postpartum, each time interval comprising 14 goats. Colostral IgG was determined by ELISA, Brix was determined by digital refractometry, and TP was determined by the Bradford protein method. Sperman’s correlations and Lin’s concordance correlation coefficient were used to determine the direction and strength of the association and to assess agreement (prediction accuracy) between methods, respectively. Receiver operator characteristic analysis was used to determine Brix and TP thresholds for predicting good-quality colostrum using several cut-offs (20, 30, 40, and 50 g/L IgG). Mean (± SD) for colostral IgG, Brix, and TP were 54 ± 22.6 g/L, 22 ± 5.0%, and 12 ± 2.8 g/dL, respectively. The statistical analysis did not provide evidence of a significant impact of time of first collection (up to 14 h postpartum), on IgG, Brix, and TP. Brix and IgG values exhibited both a high degree of correlation (r = 0.89–90) and concordance (ρc = 0.89–90), indicating a strong and reliable relationship between the two measurements. The prevalence of samples ≥ 20, 30, 40, and 50 g of IgG/L were 96, 88, 71, and 54%, respectively. Optimal Brix and TP thresholds predicting IgG ≥ 20, 30, 40, and 50 g/L IgG were 13.8, 17.5, 20.1, and 22.5%, and 6.8, 9.3, 10.8, and 11.1 g/dL, respectively. Increasing the IgG threshold resulted in lower sensitivity but higher specificity for estimating colostral IgG using Brix or TP values. The present findings indicate that delaying the first colostrum collection up to 14 h postpartum did not result in conclusive changes in colostral IgG concentration, Brix values, or total protein levels. Our results also confirm the reliability of Brix refractometry as an on-farm tool for estimating IgG concentrations in goat colostrum. These results are particularly relevant to intensive dairy systems, offering insights to enhance colostrum management and task prioritisation, especially during the bustling kidding periods.

推迟首次采集初乳:对山羊初乳中免疫球蛋白 G 的影响
本实验的动机是需要了解延迟首次采集初乳对山羊初乳中免疫球蛋白 G (IgG) 浓度的影响,以填补针对山羊研究的空白,尽管这在奶牛养殖中意义重大。同时,我们还研究了初乳 IgG、总蛋白 (TP) 和 Brix 值之间的关系。我们从 56 只萨能山羊身上采集了两份初乳样本,每半边乳房各一份。第一份样本从产后右侧乳头采集,第二份样本从产后左侧乳头采集,采集间隔为产后 0、4-6、8-10 或 12-14 小时,每个时间间隔采集 14 只山羊。用酶联免疫吸附法测定初乳 IgG,用数字折射仪测定 Brix,用 Bradford 蛋白质法测定 TP。Sperman 相关性和 Lin 一致性相关系数分别用于确定相关性的方向和强度,以及评估不同方法之间的一致性(预测准确性)。采用接收者操作特征分析法确定 Brix 和 TP 临界值,以使用几个临界值(20、30、40 和 50 克/升 IgG)预测优质初乳。初乳 IgG、Brix 和 TP 的平均值(± SD)分别为 54 ± 22.6 g/L、22 ± 5.0% 和 12 ± 2.8 g/dL。统计分析并未证明首次采集时间(产后 14 小时以内)对 IgG、Brix 和 TP 有显著影响。Brix 和 IgG 值显示出高度的相关性(r = 0.89-90)和一致性(ρc = 0.89-90),表明这两种测量值之间存在牢固可靠的关系。IgG/L≥20、30、40 和 50 克的样本比例分别为 96%、88%、71% 和 54%。预测 IgG ≥ 20、30、40 和 50 克/升的最佳 Brix 和 TP 阈值分别为 13.8%、17.5%、20.1% 和 22.5%,以及 6.8%、9.3%、10.8% 和 11.1 克/分升。提高 IgG 临界值会降低使用 Brix 或 TP 值估计初乳 IgG 的灵敏度,但会提高特异性。本研究结果表明,将首次初乳采集时间推迟到产后 14 小时并不会导致初乳 IgG 浓度、Brix 值或总蛋白水平发生确凿的变化。我们的研究结果还证实了 Brix 折光测定法作为牧场上估测山羊初乳中 IgG 浓度的工具的可靠性。这些结果与集约化奶牛场系统特别相关,为加强初乳管理和任务优先级的确定提供了启示,尤其是在热闹的产仔期。
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来源期刊
Animal
Animal 农林科学-奶制品与动物科学
CiteScore
7.50
自引率
2.80%
发文量
246
审稿时长
3 months
期刊介绍: Editorial board animal attracts the best research in animal biology and animal systems from across the spectrum of the agricultural, biomedical, and environmental sciences. It is the central element in an exciting collaboration between the British Society of Animal Science (BSAS), Institut National de la Recherche Agronomique (INRA) and the European Federation of Animal Science (EAAP) and represents a merging of three scientific journals: Animal Science; Animal Research; Reproduction, Nutrition, Development. animal publishes original cutting-edge research, ''hot'' topics and horizon-scanning reviews on animal-related aspects of the life sciences at the molecular, cellular, organ, whole animal and production system levels. The main subject areas include: breeding and genetics; nutrition; physiology and functional biology of systems; behaviour, health and welfare; farming systems, environmental impact and climate change; product quality, human health and well-being. Animal models and papers dealing with the integration of research between these topics and their impact on the environment and people are particularly welcome.
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