PD-1/PD-L1 interaction score and NKT-like cell infiltration predict immunotherapy efficacy in non-small cell lung cancer patients

IF 3.7 3区医学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Cytotherapy Pub Date : 2024-07-24 DOI:10.1016/j.jcyt.2024.07.010

Jing Zhang, Dong Lin, Huihua Hu, Haipeng Xu

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引用次数: 0

Abstract

Objective

The currently available biomarkers are insufficient to accurately predict the immunotherapy response in patients. This work attempted to investigate effects of PD-1/PD-L1 interaction score combined with NKT-like cell infiltration level in tumor microenvironment on predicting immunotherapy efficacy.

Methods

24 non-small cell lung cancer (NSCLC) patients who underwent immunotherapy were analyzed using multiplex immunofluorescence to quantitatively assess positive cells of target biomarkers and their spatial localization. Correlation between PD-1/PD-L1 interaction score in combination with NKT-like cell infiltration level and immunotherapy response was analyzed. The predictive performance of two individual biomarkers and combined novel biomarkers in immunotherapy efficacy was assessed through receiver operating characteristic curve analysis. Relationships between these factors and patient survival prognosis were analyzed using Kaplan–Meier curves.

Results

Among responders, PD-1/PD-L1 interaction score and NKT-like cell infiltration level were significantly higher than nonresponders (P < 0.05), and PD-1/PD-L1 interaction score and NKT-like cell infiltration level could effectively identify the population with immunotherapy response, with area under the curves (AUCs) of 0.7571 and 0.8643, respectively. Combination of the two had the best performance in predicting the efficacy of immunotherapy (AUC = 0.9070). High PD-1/PD-L1 interaction scores and high levels of NKT-like cell infiltration significantly improved progression-free survival (HR = 0.2544, P = 0.0053) and overall survival (HR = 0.2820, P = 0.0053) in patients.

Conclusions

Combination of PD-1/PD-L1 interaction score and NKT-like cell infiltration level had favorable performance in predicting immunotherapy response in NSCLC patients, contributing to accurately identify patients who may benefit from immunotherapy.

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PD-1/PD-L1相互作用评分和NKT样细胞浸润可预测非小细胞肺癌患者的免疫疗法疗效

目的目前可用的生物标志物不足以准确预测患者的免疫治疗反应。方法采用多重免疫荧光技术对24例接受免疫治疗的非小细胞肺癌（NSCLC）患者进行分析，定量评估目标生物标记物的阳性细胞及其空间定位。分析了 PD-1/PD-L1 相互作用评分与 NKT 样细胞浸润水平和免疫治疗反应之间的相关性。通过接收者操作特征曲线分析评估了两种单独生物标记物和组合新型生物标记物对免疫疗法疗效的预测性。结果在应答者中，PD-1/PD-L1交互作用评分和NKT样细胞浸润水平显著高于非应答者（P< 0.05），PD-1/PD-L1交互作用评分和NKT样细胞浸润水平能有效识别免疫治疗应答人群，曲线下面积（AUC）分别为0.7571和0.8643。两者的结合在预测免疫疗法疗效方面表现最佳（AUC = 0.9070）。结论PD-1/PD-L1相互作用评分高和NKT样细胞浸润水平高能显著改善患者的无进展生存期（HR = 0.2544，P = 0.0053）和总生存期（HR = 0.2820，P = 0.0053）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytotherapy 医学-生物工程与应用微生物

CiteScore

6.30

自引率

4.40%

发文量

683

审稿时长

49 days

期刊介绍： The journal brings readers the latest developments in the fast moving field of cellular therapy in man. This includes cell therapy for cancer, immune disorders, inherited diseases, tissue repair and regenerative medicine. The journal covers the science, translational development and treatment with variety of cell types including hematopoietic stem cells, immune cells (dendritic cells, NK, cells, T cells, antigen presenting cells) mesenchymal stromal cells, adipose cells, nerve, muscle, vascular and endothelial cells, and induced pluripotential stem cells. We also welcome manuscripts on subcellular derivatives such as exosomes. A specific focus is on translational research that brings cell therapy to the clinic. Cytotherapy publishes original papers, reviews, position papers editorials, commentaries and letters to the editor. We welcome "Protocols in Cytotherapy" bringing standard operating procedure for production specific cell types for clinical use within the reach of the readership.