Enhancing bioactive phenolic extraction from unfermented Cabernet Sauvignon pomace through tailored synergies of pH, proteolysis, and microwave processing

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing Pub Date : 2024-07-19 DOI:10.1016/j.fbp.2024.07.010

Sophia Pinton , Fernanda Furlan Gonçalves Dias , Larry A. Lerno , Juliana Maria Leite Nobrega de Moura Bell

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引用次数: 0

Abstract

The impact of enzyme-assisted (EAE), microwave-assisted (MAE), and microwave enzyme-assisted (MEAE) extractions using water were evaluated and compared to aqueous (AEP), conventional ethanolic (CSE), and microwave ethanolic (MSE) controls for the release of phenolics from Cabernet Sauvignon grape pomace. Optimization of extract total phenolic content (TPC) involved stepwise screening of time, temperature, slurry pH, solids-to-liquid ratio, and enzyme conditions. The use of 0.1 % alkaline protease in MEAE (1:10 g pomace/mL water, pH 11.5, 70 °C, 30 min) reduced extraction time by 50 % compared to AEP, EAE, and CSE methods, doubling the TPC of the extracts to 100.9 mg GAE/g dry weight pomace compared to ethanolic extractions. MAE and MEAE extracts exhibited in vitro antioxidant activities (ABTS and ORAC) similar to ethanolic extracts and had greater antioxidant activities than AEP/EAE extracts while boosting relative contents of catechins, procyanidins, trans-piceid, and malvidin-3,5-diglucoside as detected by untargeted metabolomics. Quantitation by HPLC showed increased levels of gallic acid, protocatechuic acid, syringic acid, p-coumaric acid, polymeric phenols, and polymeric pigments in MEAE compared to hydroethanolic methods. Scanning electron microscopy further supported the synergistic role of microwave processing and proteolysis in disrupting the grape cell structure to aid in releasing valuable bioactive phenolic compounds.

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通过 pH 值、蛋白水解和微波处理的定制协同作用，提高未发酵赤霞珠葡萄渣中生物活性酚的提取率

评估了水酶法（EAE）、微波酶法（MAE）和微波酶法（MEAE）萃取对赤霞珠葡萄渣中酚类物质释放的影响，并与水法（AEP）、传统乙醇法（CSE）和微波乙醇法（MSE）对照进行了比较。提取物总酚含量（TPC）的优化涉及时间、温度、浆液 pH 值、固液比和酶条件的逐步筛选。与AEP、EAE和CSE方法相比，在MEAE（1:10 g果渣/mL水，pH 11.5，70 °C，30分钟）中使用0.1 %的碱性蛋白酶可将萃取时间缩短50%，与乙醇萃取相比，萃取物的总酚含量翻了一番，达到100.9 mg GAE/g果渣干重。MAE 和 MEAE 提取物的体外抗氧化活性（ABTS 和 ORAC）与乙醇提取物相似，并且比 AEP/EAE 提取物具有更高的抗氧化活性，同时提高了儿茶素、原花青素、反式冰片苷和麦冬苷-3,5-二葡萄糖苷的相对含量（通过非靶向代谢组学检测）。高效液相色谱法的定量分析显示，与水乙醇法相比，MEAE 中没食子酸、原儿茶酸、丁香酸、对香豆素、聚合酚和聚合色素的含量有所增加。扫描电子显微镜进一步证实了微波处理和蛋白水解在破坏葡萄细胞结构以帮助释放有价值的生物活性酚类化合物方面的协同作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food and Bioproducts Processing 工程技术-工程：化工

CiteScore

9.70

自引率

4.30%

发文量

115

审稿时长

24 days

期刊介绍： Official Journal of the European Federation of Chemical Engineering: Part C FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering. Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing. The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those: • Primarily concerned with food formulation • That use experimental design techniques to obtain response surfaces but gain little insight from them • That are empirical and ignore established mechanistic models, e.g., empirical drying curves • That are primarily concerned about sensory evaluation and colour • Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material, • Containing only chemical analyses of biological materials.