Expression of ovine MYH3 gene and its polymorphisms and association analysis with growth traits in Hu sheep

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Kunchao Han , Fadi Li , Xiaoxue Zhang , Huibin Tian , Deyin Zhang , Kai Huang , Yukun Zhang , Yuan Zhao , Liming Zhao , Dan Xu , Jiangbo Cheng , Zongwu Ma , Xiaobin Yang , Mengru Pu , Weimin Wang
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Abstract

The growth rate of sheep determines their productivity; the faster the growth rate, the higher the productivity. Sheep growth is usually measured by body weight, which is an important indicator of overall growth, and body size, which reflects body shape and structure. Therefore, in sheep production, the selection and improvement of growth traits is one of the important means to improve production efficiency. In this study, 857 males of Hu sheep were selected to record growth traits, including body weight, height, length, and chest, and cannon circumference at 80, 100, 120, 140, 160 and 180 days of age, as well as live weight before slaughter and carcass weight. Correlation analyses conducted between these growth traits at different stages were significantly correlated with both live weight before slaughter and carcass weight. And the strongest correlation was observed for body weight, while body height showed the weakest correlation. The myosin heavy chain 3 (MYH3) gene in the MYH gene family was selected as the target gene, and a synonymous mutation (MYH3 g.33834192A>G) in the exon 12 region of MYH3 was identified by polymerase chain reaction (PCR) and Sanger sequencing. Further association analysis using competitive allele-specific PCR (KASPar) technique showed that the MYH3 g.33834192A>G locus was significantly (P < 0.05) associated with growth traits (including body weight from 80 to 160 days of age, body height at 80 and 100 days of age, body length at 100 and 140 days of age, chest circumference at 80, 100, 160, and 180 days of age, cannon circumference at 80–140 days of age and carcass weight). Among them, the dominant genotype was GG. Meanwhile, the tissue expression results indicated that the highest level of MYH3 gene expression was found in muscle tissues. Therefore, this locus can be used as a candidate molecular genetic marker for improving growth traits in Hu sheep.

绵羊 MYH3 基因的表达及其多态性以及与胡羊生长性状的关联分析
绵羊的生长速度决定其生产率;生长速度越快,生产率越高。绵羊的生长通常是通过体重和体型来衡量的,前者是衡量绵羊整体生长的重要指标,后者则反映了绵羊的体形和结构。因此,在绵羊生产中,选择和改良生长性状是提高生产效率的重要手段之一。本研究选取了 857 只胡羊的雄性,记录其 80、100、120、140、160 和 180 日龄的体重、身高、体长、胸围和炮围等生长性状,以及宰前活重和胴体重。这些生长性状在不同阶段与屠宰前活体重和胴体重都有显著的相关性。体重的相关性最强,而体高的相关性最弱。研究选择了 MYH 基因家族中的肌球蛋白重链 3(MYH3)基因作为目标基因,并通过聚合酶链式反应(PCR)和桑格测序确定了 MYH3 第 12 外显子区的同义突变(MYH3 g.33834192A>G)。利用竞争性等位基因特异性 PCR(KASPar)技术进行的进一步关联分析表明,MYH3 g.33834192A>G位点与生长性状(包括80-160日龄体重、80和100日龄体高、100和140日龄体长、80、100、160和180日龄胸围、80-140日龄炮围和胴体重)显著相关(P< 0.05)。其中,显性基因型为 GG。同时,组织表达结果表明,肌肉组织中 MYH3 基因表达水平最高。因此,该基因位点可作为改善胡羊生长性状的候选分子遗传标记。
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来源期刊
Small Ruminant Research
Small Ruminant Research 农林科学-奶制品与动物科学
CiteScore
3.10
自引率
11.10%
发文量
210
审稿时长
12.5 weeks
期刊介绍: Small Ruminant Research publishes original, basic and applied research articles, technical notes, and review articles on research relating to goats, sheep, deer, the New World camelids llama, alpaca, vicuna and guanaco, and the Old World camels. Topics covered include nutrition, physiology, anatomy, genetics, microbiology, ethology, product technology, socio-economics, management, sustainability and environment, veterinary medicine and husbandry engineering.
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