Effect of Tamarind (Tamarindus indica L.) Pulp Syrup Supplementation on the Production of Bioethanol from Sugarcane (Saccharum officinarum L.) Molasses at Different Temperatures and pH

Hussaini Madu Babale, F. Buba, N. Dibal, M. A. Milala
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Abstract

Alternative sources of environmentally friendly energy source such as biofuels are been explored with the aim of reducing environmental  pollution and cope with the growing energy demand. Bioethanol was produced from sugarcane molasses using tamarind  supplements at different pH and temperatures. The volatile profile of the bioethanol produced was evaluated using Gas  chromatography and mass spectroscopy (GC-MS). Bioethanol was produced from sugarcane molasses hydrolyzed using tamarind pulp  syrup (TPS) and distilled water (DW) at different temperatures (26-30 oC) and pH (4.5-6.5) and utilizing Saccharomyces cerevisiae for  fermentation. The results showed a significant increase in reducing sugar yield, bioethanol yield/quantity, and bioethanol volatility for the  bioethanol produced using TPS hydrolysis compared to those produced using DW (p<0.05). The suitable temperature and pH for the  production of the maximum amount of reducing sugar and bioethanol were 28 oC and 5.5 respectively. The volatility of the bioethanol  produced was highest at a temperature of 28 oC and a pH of 5.5. The density of bioethanol obtained using TPS (0.797 g/cm3) was close to  the standard density of bioethanol (0.789 g/cm3). Conclusively, TPS was found to enhance bioethanol production from sugarcane  molasses through fermentation. This suggests that it could serve as an alternative hydrolyzer for the production of biofuel.  
不同温度和 pH 值下添加罗望子(Tamarindus indica L.)浆对甘蔗(Saccharum officinarum L.)糖蜜生产生物乙醇的影响
为了减少环境污染和满足日益增长的能源需求,人们一直在探索生物燃料等环保能源的替代来源。在不同的 pH 值和温度下,使用罗望子补充剂从甘蔗糖蜜中生产生物乙醇。使用气相色谱和质谱(GC-MS)对所生产的生物乙醇的挥发性进行了评估。使用罗望子糖浆(TPS)和蒸馏水(DW)在不同温度(26-30 oC)和 pH 值(4.5-6.5)下水解甘蔗糖蜜,并利用酿酒酵母发酵生产生物乙醇。结果表明,与使用 DW 生产的生物乙醇相比,使用 TPS 水解生产的生物乙醇在还原糖产量、生物乙醇产量/数量和生物乙醇挥发性方面均有显著提高(p<0.05)。产生最多还原糖和生物乙醇的合适温度和 pH 值分别为 28 摄氏度和 5.5。温度为 28 摄氏度、pH 值为 5.5 时生产的生物乙醇挥发性最高。使用 TPS 获得的生物乙醇密度(0.797 克/立方厘米)接近生物乙醇的标准密度(0.789 克/立方厘米)。结论是,TPS 可以通过发酵提高甘蔗糖蜜的生物乙醇产量。这表明它可以作为生产生物燃料的替代水解器。
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