Enterobacter hormaechei Bacteria from Paper Napkins Confirmed by 16S rRNA Gene and Experimental Infection in Rats

Abstract

Background: With the frequent use of paper napkins for everyday personal use and direct contact with the external openings of our bodies such as the nostril, eyes and even the vagina. With its many types and the lack of necessary sterilization methods in factories, it is likely that it contains germs with the possibility of entering them. The current study aimed isolated E. hormaechei to experimentally infected and determine the pathogenicity of E. hormaechei in rat newborns and adult rats by two ways of infection. Methods: Confirmed by this unique research with its content by isolating bacteria Enterobacter hormaechei at a large presence rate per 0.2 mg of each napkin. In humans, Enterobacter hormaechei normally behaves as an opportunistic pathogenic bacteria. Vitek 2 compact biochemical tests were performed on fifty E. hormaechei isolates. Result: Three strains of E. hormaechei that were examined and closely linked to Enterobacter spp. had their 16S rRNA gene sequenced using the Sanger method. The bacteria’s closest relatives among Gen Bank sequences were Enterobacter cloacae and Enterobacter hormaechei. (99-100%). Interstitial pneumonia, acute enteritis, necrosis and inflammation-related cell infiltration in the parenchyma of the uterus and atretic oviduct epithelium are all seen in the histopathological examination of affected rats.