A Cartridge Structure Facilitates Clinical Mass Spectrometry Pretreatment and Can be Integrated Into a High‐Throughput Instrument

IF 1.3 Q4 CHEMISTRY, ANALYTICAL
Xiaofei He, Mengxue Feng, Xiaofeng Xu, Hong Zhao, Xueqing Qian
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引用次数: 0

Abstract

Liquid chromatography–triple quadrupole mass spectrometry (LC–MS/MS) is becoming an increasingly essential analytical technique in many fields, such as food, environmental, biochemical, pharmaceutical, and clinical chemistry. The testing matrices, such as plasma and serum, cannot usually be sent directly to the LC–MS/MS system because of the large number of unrelated substances in addition to target analytes in samples, such as cells, tissues, and proteins. Protein precipitation (PPT) is the most utilized pretreatment method in clinical analysis because of its ease of use; its performance always involves the separation of the liquid phase from the solid phase, in which the most commonly used manipulation is centrifugation. However, there is an upper limit of throughput for centrifugation, and the cost and difficulty of integrating centrifugation into automatic equipment are usually high. To solve the drawbacks of the current PPT method, we developed a cartridge structure that can omit the centrifugation step in PPT, which, in turn, can facilitate the incorporation of the PPT method into an automatic clinical pretreatment procedure for LC–MS/MS. We used vitamin D analysis as a representative application in our endeavor to develop a centrifugation‐free PPT method that can be easily applied to a new automatic clinical pretreatment procedure.
盒式结构有助于临床质谱前处理,并可集成到高通量仪器中
液相色谱-三重四极杆质谱(LC-MS/MS)正日益成为食品、环境、生化、制药和临床化学等许多领域必不可少的分析技术。血浆和血清等检测基质通常不能直接送入 LC-MS/MS 系统,因为样品中除了目标分析物外,还有大量不相关的物质,如细胞、组织和蛋白质。蛋白质沉淀(PPT)是临床分析中最常用的前处理方法,因为它使用方便;其操作过程总是涉及液相与固相的分离,其中最常用的操作是离心。然而,离心法的处理量有上限,而且将离心法集成到自动设备中的成本和难度通常很高。为了解决目前 PPT 方法的弊端,我们开发了一种滤芯结构,可以省略 PPT 中的离心步骤,进而便于将 PPT 方法纳入 LC-MS/MS 的临床自动前处理程序。我们以维生素 D 分析为代表,努力开发一种无需离心的 PPT 方法,使其能方便地应用于新的自动临床前处理程序。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
SEPARATION SCIENCE PLUS
SEPARATION SCIENCE PLUS CHEMISTRY, ANALYTICAL-
CiteScore
1.90
自引率
9.10%
发文量
111
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