Effect of Camellia flava (Pitard) Sealy flower extract on the degeneration of Islets of Langerhans and insulin resistance in alloxan-induced hyperglycemia model on Swiss albino mice

Huu Lac Thuy Nguyen, Thi Thu Phuong Ha, Ngoc Phuc Nguyen, Nguyen Hoang Linh Phan, Dang Thuy Hien Nguyen, Van Dat Truong, Minh-Nhut Truong, Thao-My Nguyen-Hoang, Thi Nghia Luu, Ngoc Phuc Nguyen, Thanh Binh Nguyen, H. Mai
{"title":"Effect of Camellia flava (Pitard) Sealy flower extract on the degeneration of Islets of Langerhans and insulin resistance in alloxan-induced hyperglycemia model on Swiss albino mice","authors":"Huu Lac Thuy Nguyen, Thi Thu Phuong Ha, Ngoc Phuc Nguyen, Nguyen Hoang Linh Phan, Dang Thuy Hien Nguyen, Van Dat Truong, Minh-Nhut Truong, Thao-My Nguyen-Hoang, Thi Nghia Luu, Ngoc Phuc Nguyen, Thanh Binh Nguyen, H. Mai","doi":"10.3897/pharmacia.71.e126255","DOIUrl":null,"url":null,"abstract":"Diabetes has always been a matter of concern to health experts as well as the community due to the increasing number of patients with diabetes and the severe consequences it may cause. Many attempts have been made to discover new treatment options for diabetes, and herbal medicines are currently considered to have great potential. This study was conducted to evaluate the effect of Camellia flava flower extract on the degeneration of the islets of Langerhans and insulin resistance in an alloxan-induced hyperglycemia model in Swiss albino mice. Hyperglycemic conditions were induced by alloxan (55 mg/kg, i.v.). The animals were then treated with glibenclamide (10 mg/kg, p.o.) and flower extract at doses of 1.09 and 2.19 g/kg, p.o. The results showed that the blood glucose, AUC, HbA1c, and HOMA-IR levels of two groups of mice receiving flower extract were considerably lower than those of the hyperglycemic untreated group (p < 0.05). The body weights of these two groups were also lower than the untreated group on the last day of the experiment, though the differences were not significant (p > 0.05). However, this was not observed when assessing insulin levels as well as relative organ weights. In biochemical tests, creatinine and AST and ALT concentrations were evaluated. There was no significant variation in creatinine and AST concentrations between the five experimental groups, whereas mice treated with glibenclamide and flower extract at both doses showed a remarkable decline in ALT concentration (p < 0.05). The hepatic histomicrographs were consistent with ALT results, while the H&E staining of kidneys showed no difference between groups. Histomicrographs of the pancreas revealed that the treatment groups using glibenclamide and flower extract had larger islets of Langerhans than those of the alloxan-treated group. Based on these results, this study demonstrated that Camellia flava flower extract exerted several beneficial effects, including blood sugar level reduction, weight loss promotion, and organ protection, hence making it a new potential herbal medication for the management of diabetes.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3897/pharmacia.71.e126255","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Diabetes has always been a matter of concern to health experts as well as the community due to the increasing number of patients with diabetes and the severe consequences it may cause. Many attempts have been made to discover new treatment options for diabetes, and herbal medicines are currently considered to have great potential. This study was conducted to evaluate the effect of Camellia flava flower extract on the degeneration of the islets of Langerhans and insulin resistance in an alloxan-induced hyperglycemia model in Swiss albino mice. Hyperglycemic conditions were induced by alloxan (55 mg/kg, i.v.). The animals were then treated with glibenclamide (10 mg/kg, p.o.) and flower extract at doses of 1.09 and 2.19 g/kg, p.o. The results showed that the blood glucose, AUC, HbA1c, and HOMA-IR levels of two groups of mice receiving flower extract were considerably lower than those of the hyperglycemic untreated group (p < 0.05). The body weights of these two groups were also lower than the untreated group on the last day of the experiment, though the differences were not significant (p > 0.05). However, this was not observed when assessing insulin levels as well as relative organ weights. In biochemical tests, creatinine and AST and ALT concentrations were evaluated. There was no significant variation in creatinine and AST concentrations between the five experimental groups, whereas mice treated with glibenclamide and flower extract at both doses showed a remarkable decline in ALT concentration (p < 0.05). The hepatic histomicrographs were consistent with ALT results, while the H&E staining of kidneys showed no difference between groups. Histomicrographs of the pancreas revealed that the treatment groups using glibenclamide and flower extract had larger islets of Langerhans than those of the alloxan-treated group. Based on these results, this study demonstrated that Camellia flava flower extract exerted several beneficial effects, including blood sugar level reduction, weight loss promotion, and organ protection, hence making it a new potential herbal medication for the management of diabetes.
山茶花提取物对阿洛糖诱导的瑞士白化小鼠高血糖模型中朗格汉斯胰岛退化和胰岛素抵抗的影响
由于糖尿病患者人数不断增加及其可能造成的严重后果,糖尿病一直是健康专家和社会关注的问题。人们已经做了很多尝试来发现治疗糖尿病的新方法,而草药目前被认为具有很大的潜力。本研究旨在评估山茶花提取物对阿脲诱导的瑞士白化小鼠高血糖模型中朗格汉斯胰岛退化和胰岛素抵抗的影响。阿脲(55 毫克/千克,静脉注射)诱导高血糖。结果表明,接受花提取物治疗的两组小鼠的血糖、AUC、HbA1c 和 HOMA-IR 水平大大低于未接受治疗的高血糖组(P < 0.05)。在实验的最后一天,这两组小鼠的体重也低于未处理组,但差异不显著(P > 0.05)。然而,在评估胰岛素水平和相对器官重量时却没有观察到这种情况。在生化测试中,评估了肌酐、谷草转氨酶和谷丙转氨酶的浓度。五个实验组的肌酐和谷草转氨酶浓度没有明显变化,而使用格列本脲和花提取物两种剂量的小鼠的谷丙转氨酶浓度明显下降(p < 0.05)。肝脏组织显微照片与 ALT 结果一致,而肾脏的 H&E 染色结果显示组间无差异。胰腺组织显微照片显示,格列本脲和花提取物治疗组的朗格汉斯小体大于阿脲治疗组。基于这些结果,本研究证明了山茶花提取物具有多种有益作用,包括降低血糖水平、促进减肥和保护器官,因此它是一种潜在的治疗糖尿病的新草药。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信