Application of [2-((Z)-(4-hydroxy-3-methoxy-5-((E)-thiazol-5-yldiazenyl) benzenylidene) amino) benzoic acid] (MThBABA) in Extractive Spectrophotometric Determination of Copper (II)

Abstract

For estimation of copper at the trace level a simple, sensitive and rapid spectrophotometric method has been developed using MTHBABA reagent. Elemental and spectral analysis techniques were used for characterization of MTHBABA reagent. Solvent chloroform was used for quantitative (99.61 %) extraction of Copper (II) with the help of MTHBABA at pH range 5.6 to 6.7 from aqueous solution. An intense peak of λ max (540 nm) was observed from chloroform extract. Concentration range for Beers law observed between 2.5 to 30 µg/ml for Copper (II). For Copper complex MTHBABA molar absorptivity and sandell’s sensitivity was found to be 39524 L mole-1cm-1 and 0.0243 μgcm-2 respectively. Job’s Continuous Variation as well as the Mole Ratio Method confirm complex nature 1:1 (Copper: MTHBABA). During MTHBABA present study interference of various ions is also discussed. The proposed method has been successfully applied for determination of Copper (II) in alloy and pharmaceutical samples