Evaluation of antibacterial property and phytochemical composition of the crude ethanolic extract from Kalingag (Cinnamomum mercadoi) barks

Maxime Benedict Paguirigan Tirol, Francis Augustine Autor Bajao, Pamela Guillena Biwang, Joenly Rose Avendaño Cardenas, Al-Farok Calandada Casidar, John Miguel Petallar Chiong, Sweet Elise Pelaez Labnao, Jovelyn Laurente Lacubtan, Hanagen Liclican Salvador, Madeleine Cabeltes Zamayla, Jevie Lyn Peralta-Tan Nery, Shosi Mari Salubre Larido, Sandra Enriquez Laguimun, Rowel Paningbatan Catchillar, Justin Dave Magracia Manantan, Jan Karlo Tiongson Ecalne
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Abstract

Cinnamomum mercadoi, locally known as Kalingag, is a native plant of the Philippines that has limited studies despite its long use in traditional Philippine medicine due to its indigenous origin. This study examined the phytochemical composition and antibacterial activities of a crude ethanolic extract derived from Kalingag barks. The plant bark gathered from Davao Oriental was thoroughly rinsed with distilled water to eliminate any attached dirt, oven-dried for 6 hours, and then pulverized using a Wiley mill. The bark was then subjected to Soxhlet extraction, and the pooled extracts were concentrated for 5 hours at 40°C to produce a green, syrupy substance. The extract was tested qualitatively, followed by FTIR analysis to confirm the results, before being subjected to the agar well diffusion method to evaluate its antibacterial properties against Staphylococcus aureus and Escherichia coli. The phytochemical evaluation and subsequent FTIR analysis revealed that the extract contained various phytochemicals, including glycosides, tannins, phytosterols, and terpenoids. The antibacterial testing revealed concentration-dependent inhibition that could be improved by purifying or isolating specific compounds from the extract. Additional research into other plant parts is suggested to enhance its biomedical and therapeutic applications.
评估卡林格(Cinnamomum mercadoi)树皮粗乙醇提取物的抗菌特性和植物化学成分
Cinnamomum mercadoi(当地人称作 Kalingag)是菲律宾的一种本地植物,尽管因其原产地而长期用于菲律宾传统医学,但对它的研究却很有限。本研究考察了从 Kalingag 树皮中提取的粗乙醇提取物的植物化学成分和抗菌活性。从东达沃采集的植物树皮用蒸馏水彻底冲洗以去除附着的污物,烘干 6 小时,然后用威利磨粉碎。然后对树皮进行索氏萃取,在 40°C 下浓缩 5 小时,得到绿色糖浆状物质。对提取物进行定性测试,然后进行傅立叶变换红外光谱分析以确认结果,最后采用琼脂井扩散法评估其对金黄色葡萄球菌和大肠杆菌的抗菌特性。植物化学评估和随后的傅立叶变换红外分析表明,萃取物中含有多种植物化学物质,包括苷、单宁、植物甾醇和萜类化合物。抗菌测试显示,抑制作用与浓度有关,可以通过纯化或分离提取物中的特定化合物来改善抑制作用。建议对其他植物部分进行更多研究,以提高其生物医学和治疗应用。
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