{"title":"Ultrathin Polyelectrolyte Multilayer Films with Silver Nanoparticles as a Potential Antibacterial Coating","authors":"Meluzvia Marie Amora, N. G. Fundador, E. Fundador","doi":"10.12982/cmjs.2024.061","DOIUrl":null,"url":null,"abstract":"Silv er nanoparticles (AgNPs) are already used as antibacterial agents for medical devices and food packaging materials. However, concerns about its toxicity still exist. AgNPs can be incorporated into ultrathin polyelectrolyte multilayer (PEM) films to minimize this risk. With PEMs, full surface coverage can be achieved with minimal AgNPs, minimizing human exposure. In this study, a simple protocol to fabricate ultrathin PEM/AgNP films using sodium ascorbate as a reductant was developed. The effects of the number of bilayers and ionic strength (0.05 to 0.50 M) on film thickness and amount of AgNPs were investigated. UV/VIS spectra show that increasing the number of bilayers from 1 to 5 would lead to a corresponding increase in film thickness and amount of synthesized AgNPs. Thicker layers and more AgNPs were deposited when the films were fabricated under higher ionic strengths. Films with thicknesses ranging from 15.67 to 87.13 nm were fabricated. The sizes of the incorporated AgNPs were between 54.32 and 259.26 nm, as characterized by scanning electron microscopy. All films were stable when submerged in water for 240 h, suggesting the migration of Ag/Ag+ from the films was minimal. The antibacterial efficacy of the films against Staphylococcus aureus was also assessed. Only the PEM/AgNP films fabricated with the highest salt concentration (0.50 M) showed antibacterial activity under resazurin assay. However, the Kirby Bauer method showed inhibition zones for all films fabricated under all salt concentrations, indicating antibacterial activity at the interface of the bacterial lawn and the films, even with the slow migration of Ag/Ag+. Thus, PEM/AgNP films have the potential to be used as an antibacterial coating.","PeriodicalId":9884,"journal":{"name":"Chiang Mai Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chiang Mai Journal of Science","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.12982/cmjs.2024.061","RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Silv er nanoparticles (AgNPs) are already used as antibacterial agents for medical devices and food packaging materials. However, concerns about its toxicity still exist. AgNPs can be incorporated into ultrathin polyelectrolyte multilayer (PEM) films to minimize this risk. With PEMs, full surface coverage can be achieved with minimal AgNPs, minimizing human exposure. In this study, a simple protocol to fabricate ultrathin PEM/AgNP films using sodium ascorbate as a reductant was developed. The effects of the number of bilayers and ionic strength (0.05 to 0.50 M) on film thickness and amount of AgNPs were investigated. UV/VIS spectra show that increasing the number of bilayers from 1 to 5 would lead to a corresponding increase in film thickness and amount of synthesized AgNPs. Thicker layers and more AgNPs were deposited when the films were fabricated under higher ionic strengths. Films with thicknesses ranging from 15.67 to 87.13 nm were fabricated. The sizes of the incorporated AgNPs were between 54.32 and 259.26 nm, as characterized by scanning electron microscopy. All films were stable when submerged in water for 240 h, suggesting the migration of Ag/Ag+ from the films was minimal. The antibacterial efficacy of the films against Staphylococcus aureus was also assessed. Only the PEM/AgNP films fabricated with the highest salt concentration (0.50 M) showed antibacterial activity under resazurin assay. However, the Kirby Bauer method showed inhibition zones for all films fabricated under all salt concentrations, indicating antibacterial activity at the interface of the bacterial lawn and the films, even with the slow migration of Ag/Ag+. Thus, PEM/AgNP films have the potential to be used as an antibacterial coating.
期刊介绍:
The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.