Realita Dini Mustika, N. H. Aprilita, Dwi Siswanta
{"title":"The Development of Digital Colorimetric-Based Thiourea Receptor for Highly Sensitive Detection of Dihydrogen Phosphate","authors":"Realita Dini Mustika, N. H. Aprilita, Dwi Siswanta","doi":"10.12982/cmjs.2024.056","DOIUrl":null,"url":null,"abstract":"T his study aimed to synthesize a new chemosensor, namely 1,1’-(1,4-phenylene)bis(3-(4-nitrophenyl)thiourea), specifically designed for detection of H2PO4−. The compound was characterized using FTIR, LC-MS, 1H NMR, and 13C NMR, while the colorimetric sensing abilities were validated through various analyses. These include linearity, limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy using UV-Vis spectrophotometry, RGB, and HSV color components. Digital images of color changes were captured using a smartphone camera and Color Grab app was used to quantify color components. Subsequently, anions test (NO3−, Cl–, HSO4−, and H2PO4−) were conducted in acetonitrile, where the addition of H2PO4− induced a distinct color change from colorless to yellow. The results showed that detection of H2PO4− depended on pH of the solution, with no color change observed at pH 1-3 due to anions converting to acidic species. At pH 5, H2PO4− was detected as a yellow color change, and alkaline conditions led to different color responses. Furthermore, UV-Vis detection showed recovery values of 99.80% to 100.6%, indicating high accuracy. RGB and HSV color component-based sensors showed recovery values of 101.6% to 114.4% and 92.50% to 110.2%, respectively. The LOD of the UV-Vis, RGB, and HSV methods were 0.16 μM, 0.46 μM, and 0.18 μM, while LOQ values were 0.54 μM,1.52 μM, and 0.61 μM, respectively. In conclusion, the synthesized chemosensor showed potential for accurate and sensitive detection of H2PO4− using UV-Vis spectrophotometry and digital colorimetric-based image.","PeriodicalId":9884,"journal":{"name":"Chiang Mai Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chiang Mai Journal of Science","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.12982/cmjs.2024.056","RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
T his study aimed to synthesize a new chemosensor, namely 1,1’-(1,4-phenylene)bis(3-(4-nitrophenyl)thiourea), specifically designed for detection of H2PO4−. The compound was characterized using FTIR, LC-MS, 1H NMR, and 13C NMR, while the colorimetric sensing abilities were validated through various analyses. These include linearity, limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy using UV-Vis spectrophotometry, RGB, and HSV color components. Digital images of color changes were captured using a smartphone camera and Color Grab app was used to quantify color components. Subsequently, anions test (NO3−, Cl–, HSO4−, and H2PO4−) were conducted in acetonitrile, where the addition of H2PO4− induced a distinct color change from colorless to yellow. The results showed that detection of H2PO4− depended on pH of the solution, with no color change observed at pH 1-3 due to anions converting to acidic species. At pH 5, H2PO4− was detected as a yellow color change, and alkaline conditions led to different color responses. Furthermore, UV-Vis detection showed recovery values of 99.80% to 100.6%, indicating high accuracy. RGB and HSV color component-based sensors showed recovery values of 101.6% to 114.4% and 92.50% to 110.2%, respectively. The LOD of the UV-Vis, RGB, and HSV methods were 0.16 μM, 0.46 μM, and 0.18 μM, while LOQ values were 0.54 μM,1.52 μM, and 0.61 μM, respectively. In conclusion, the synthesized chemosensor showed potential for accurate and sensitive detection of H2PO4− using UV-Vis spectrophotometry and digital colorimetric-based image.
期刊介绍:
The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.