Antimicrobial Activity of Biosynthesized Copper Nanoparticles Using Methanolic Extract of Ocimum Sanctum

Abstract

Abstract: Methanolic extract of Ocimum sanctum leaves were used as a reducing and stabilizing agent for the synthesis of copper nanoparticles (CuNPs). It is a cost-effective and eco-friendly process. On the treatment of Ocimum sanctum leaf extract with copper sulphate solution, stable CuNPs were formed. The formed CuNPs were characterized under UV-Vis spectrophotometer. The biologically synthesized copper nanoparticles show high antibacterial activity against opportunistic pathogen Staphylococcus aureus. The antimicrobial activity was determined by three assays with agar well diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) and the values were compared to observe the antimicrobial efficacy of the CuNPs.