Sodium copper chlorophyllin-loaded chitosan nanoparticle-based photodynamic therapy for B16 melanoma cancer cells

IF 3.2 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Vinod Ravasaheb Shinde, Sajmina Khatun, Ajinkya Madhukar Thanekar, Basu Bhattacharjee, Aravind Kumar Rengan
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Abstract

Melanoma is one of the most aggressive and fatal skin cancers owing to its ability to metastasize and develop resistance to chemotherapy. Photodynamic therapy (PDT) is a minimally noninvasive treatment modality comprising photosensitizers (PSs), light sources, and endogenous molecular oxygen that exert a localized cytotoxic effect on cancer cells. The current study explores the therapeutic potential of sodium copper chlorophyllin-loaded chitosan nanoparticles (CH-SCC NPs) along with handheld laser-based PDT on B16 cancer cells. A modified chlorophyll derivative identified as sodium copper chlorophyllin (SCC) is a dietary supplement that has anticancer properties. Herein, we have synthesized CH-SCC NPs using the ionic gelation method to enhance the PS's bioavailability and efficiency. Chitosan nanoparticles exhibited high biocompatibility in a normal cell line L929, zebrafish, and chick embryos, and were successfully employed to deliver the SCC to cancer cells. CH-SCC NPs showed an enhanced PDT effect that killed approximately 80%–85% of B16 cells. CH-SCC NPs in combination with a handheld portable laser source showed significant therapeutic potential against the B16 skin cancer cell line. The experimental findings further strengthen our device-repurposing strategy, which suggests that SCC nanoformulations along with handheld laser can be a suitable treatment for skin cancer even in remote areas where power source and treatment cost can be a limitation.

Abstract Image

Abstract Image

基于壳聚糖纳米粒子的 B16 黑色素瘤细胞光动力疗法。
黑色素瘤是最具侵袭性和致命性的皮肤癌之一,因为它具有转移和对化疗产生抗药性的能力。光动力疗法(PDT)是一种微创无创治疗方式,由光敏剂、光源和内源性分子氧组成,可对癌细胞产生局部细胞毒性作用。本研究探讨了叶绿素钠铜壳聚糖纳米粒子(CH-SCC NPs)与手持激光光导疗法对 B16 癌细胞的治疗潜力。改性叶绿素衍生物叶绿素铜钠(SCC)是一种具有抗癌特性的膳食补充剂。在此,我们采用离子凝胶法合成了 CH-SCC NPs,以提高 PS 的生物利用度和效率。壳聚糖纳米颗粒在正常细胞系 L929、斑马鱼和小鸡胚胎中表现出很高的生物相容性,并被成功地用于向癌细胞递送 SCC。CH-SCC NPs 显示出增强的 PDT 效果,可杀死约 80%-85% 的 B16 细胞。CH-SCC NPs 与手持便携式激光源结合使用,对 B16 皮肤癌细胞株具有显著的治疗潜力。这些实验结果进一步加强了我们的设备再利用战略,表明 SCC 纳米制剂与手持激光器结合可用于治疗皮肤癌,即使是在电源和治疗成本受到限制的偏远地区也是如此。
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来源期刊
Chemical Biology & Drug Design
Chemical Biology & Drug Design 医学-生化与分子生物学
CiteScore
5.10
自引率
3.30%
发文量
164
审稿时长
4.4 months
期刊介绍: Chemical Biology & Drug Design is a peer-reviewed scientific journal that is dedicated to the advancement of innovative science, technology and medicine with a focus on the multidisciplinary fields of chemical biology and drug design. It is the aim of Chemical Biology & Drug Design to capture significant research and drug discovery that highlights new concepts, insight and new findings within the scope of chemical biology and drug design.
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