Preservation of urine specimens for metabolic evaluation of recurrent urinary stone formers.

IF 3.8 2区医学 Q1 MEDICAL LABORATORY TECHNOLOGY

Clinical chemistry and laboratory medicine Pub Date : 2024-07-30 DOI:10.1515/cclm-2024-0773

Tomáš Šálek, Pavel Musil, Pieter Vermeersch, Rachel Marrington, Zeliha G Dikmen, Radka Poláchová, Ulrike Kipman, Timo T Kouri, Janne Cadamuro

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引用次数: 0

Abstract

Objectives: Stability of concentrations of urinary stone-related metabolites was analyzed from samples of recurrent urinary stone formers to assess necessity and effectiveness of urine acidification during collection and storage.

Methods: First-morning urine was collected from 20 adult calcium-stone forming patients at Tomas Bata Hospital in the Czech Republic. Urine samples were analyzed for calcium, magnesium, inorganic phosphate, uric acid, sodium, potassium, chloride, citrate, oxalate, and urine particles. The single-voided specimens were collected without acidification, after which they were divided into three groups for storage: samples without acidification ("NON"), acidification before storage ("PRE"), or acidification after storage ("POST"). The analyses were conducted on the day of arrival (day 0, "baseline"), or after storage for 2 or 7 days at room temperature. The maximum permissible difference (MPD) was defined as ±20 % from the baseline.

Results: The urine concentrations of all stone-related metabolites remained within the 20 % MPD limits in NON and POST samples after 2 days, except for calcium in NON sample of one patient, and oxalate of three patients and citrate of one patient in POST samples. In PRE samples, stability failed in urine samples for oxalate of three patients, and for uric acid of four patients after 2 days. Failures in stability often correlated with high baseline concentrations of those metabolites in urine.

Conclusions: Detailed procedures are needed to collect urine specimens for analysis of urinary stone-related metabolites, considering both patient safety and stability of those metabolites. We recommend specific preservation steps.

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保存尿液标本，用于对复发性尿路结石患者进行代谢评估。

目的：分析复发性尿结石患者尿液中结石相关代谢物浓度的稳定性：分析复发性尿结石患者尿液中结石相关代谢物浓度的稳定性，以评估尿液酸化在收集和储存过程中的必要性和有效性：方法：从捷克共和国托马斯-巴塔医院（Tomas Bata Hospital）的20名成年钙结石患者中收集晨尿。对尿液样本进行了钙、镁、无机磷酸盐、尿酸、钠、钾、氯化物、柠檬酸盐、草酸盐和尿液颗粒分析。采集的单排标本未经酸化处理，然后分为三组储存：未经酸化处理（"NON"）、储存前酸化处理（"PRE"）或储存后酸化处理（"POST"）。分析在到达当天（第 0 天，"基线"）或在室温下储存 2 天或 7 天后进行。最大允许差值（MPD）定义为与基线值的±20%：结果：2 天后，除了一名患者的非基线样本中的钙以及三名患者的草酸盐和一名患者的枸橼酸盐外，所有与结石有关的代谢物在非基线样本和前置样本中的尿液浓度均保持在 20% 的最大允许差值范围内。在预处理样本中，3 名患者尿样中的草酸盐和 4 名患者尿样中的尿酸在 2 天后稳定性失效。稳定性失效往往与尿液中这些代谢物的基线浓度较高有关：结论：收集尿液标本以分析尿结石相关代谢物需要详细的程序，既要考虑患者的安全，也要考虑这些代谢物的稳定性。我们建议采取具体的保存步骤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical chemistry and laboratory medicine 医学-医学实验技术

CiteScore

11.30

自引率

16.20%

发文量

306

审稿时长

3 months

期刊介绍： Clinical Chemistry and Laboratory Medicine (CCLM) publishes articles on novel teaching and training methods applicable to laboratory medicine. CCLM welcomes contributions on the progress in fundamental and applied research and cutting-edge clinical laboratory medicine. It is one of the leading journals in the field, with an impact factor over 3. CCLM is issued monthly, and it is published in print and electronically. CCLM is the official journal of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) and publishes regularly EFLM recommendations and news. CCLM is the official journal of the National Societies from Austria (ÖGLMKC); Belgium (RBSLM); Germany (DGKL); Hungary (MLDT); Ireland (ACBI); Italy (SIBioC); Portugal (SPML); and Slovenia (SZKK); and it is affiliated to AACB (Australia) and SFBC (France). Topics: - clinical biochemistry - clinical genomics and molecular biology - clinical haematology and coagulation - clinical immunology and autoimmunity - clinical microbiology - drug monitoring and analysis - evaluation of diagnostic biomarkers - disease-oriented topics (cardiovascular disease, cancer diagnostics, diabetes) - new reagents, instrumentation and technologies - new methodologies - reference materials and methods - reference values and decision limits - quality and safety in laboratory medicine - translational laboratory medicine - clinical metrology Follow @cclm_degruyter on Twitter!