Immunohistochemical characteristics of cytokeratin expression in epithelial type thymoma and thymic epithelial hyperplasia in F344 rats

IF 0.9 4区医学 Q4 PATHOLOGY

Journal of Toxicologic Pathology Pub Date : 2024-07-25 DOI:10.1293/tox.2024-0045

Yuki TOMONARI, Junko SATO, Mitsutoshi UCHIDA, Takeshi KANNO, Takuya DOI, Yoshiyasu KOBAYASHI

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Abstract

We have previously reported on thymomas in Wistar Hannover rats with medullary differentiation and revealed that two different cytokeratin (CK) immunohistochemical types of thymic epithelia (TE), CK18 and CK14, lead to the formation of cortical-medullary structures. In aged F344 rats, epithelial type thymoma rarely occurs, and thymic epithelial hyperplasia is common. However, CK expression in these F344 rat lesions is unknown. We investigated three hyperplasia and four thymomas in F344 for histopathological features and CK18 and CK14 expression. Hyperplasia was characterized by an increase in tubular structures in the medulla. Thymomas were nodular in shape, with tubular structures similar to those observed in hyperplasia, along with irregular structures such as cord, papillary, and spindloid. Immunohistochemical analysis revealed that the tubular structures consisted of two layers: inner cuboidal-to-columnar TE and outer round-to-oval TE, positive for CK18 and CK14, respectively. The two-layer pattern was maintained to some extent in the irregular structures.

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F344 大鼠上皮型胸腺瘤和胸腺上皮增生中细胞角蛋白表达的免疫组化特征

我们以前曾报道过具有髓质分化的 Wistar Hannover 大鼠胸腺瘤，并揭示了胸腺上皮（TE）的两种不同细胞角蛋白（CK）免疫组化类型（CK18 和 CK14）会导致皮质-髓质结构的形成。在老年 F344 大鼠中，上皮型胸腺瘤很少发生，胸腺上皮增生很常见。然而，CK 在这些 F344 大鼠病变中的表达尚不清楚。我们研究了三例F344大鼠胸腺上皮增生和四例胸腺瘤的组织病理学特征以及CK18和CK14的表达。增生的特点是髓质中管状结构增多。胸腺瘤呈结节状，其管状结构与增生症中观察到的结构相似，同时还有不规则结构，如索状、乳头状和纺锤状。免疫组化分析显示，管状结构由两层组成：内层为立方体至柱状TE，外层为圆形至椭圆形TE，CK18和CK14分别呈阳性。不规则结构在一定程度上保持了两层模式。

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来源期刊

Journal of Toxicologic Pathology PATHOLOGY-TOXICOLOGY

CiteScore

2.10

自引率

16.70%

发文量

审稿时长

>12 weeks

期刊介绍： JTP is a scientific journal that publishes original studies in the field of toxicological pathology and in a wide variety of other related fields. The main scope of the journal is listed below. Administrative Opinions of Policymakers and Regulatory Agencies Adverse Events Carcinogenesis Data of A Predominantly Negative Nature Drug-Induced Hematologic Toxicity Embryological Pathology High Throughput Pathology Historical Data of Experimental Animals Immunohistochemical Analysis Molecular Pathology Nomenclature of Lesions Non-mammal Toxicity Study Result or Lesion Induced by Chemicals of Which Names Hidden on Account of the Authors Technology and Methodology Related to Toxicological Pathology Tumor Pathology; Neoplasia and Hyperplasia Ultrastructural Analysis Use of Animal Models.