Lymphatic Capillarization in Different Fiber Types of Rat Skeletal Muscles With Growth and Age

IF 1.9 4区医学 Q3 HEMATOLOGY

Microcirculation Pub Date : 2024-07-27 DOI:10.1111/micc.12879

Yoshikazu Taketa, Keigo Tamakoshi, Kazuki Hotta, Shutaro Maki, Toru Taguchi, Hideaki Takahashi

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Abstract

Objective

To clarify the effect of growth and advancing age on lymphatic capillarization in rat skeletal muscles, we examined the histological and biochemical changes of lymphatic capillaries in different fiber types of skeletal muscles across juvenile, young, and middle-aged generations.

Methods

We collected the tibialis anterior (TA), extensor digitorum longus (EDL), and soleus (SOL) muscles. Immunohistochemical staining using LYVE-1 and CD31 markers was used for lymphatic and blood capillaries, respectively. Real-time PCR was used to analyze mRNA expression of lymphangiogenic factors.

Results

The density of LYVE-1-positive lymphatic capillaries in the muscles peaked during the juvenile period and subsequently decreased with increasing age. In contrast to blood capillaries, fast-twitch dominant muscles (i.e., TA and EDL) exhibited an age-related decrease in lymphatic capillaries. Similar to blood capillaries, lymphatic capillaries were abundant in SOL, a slow-twitch dominant muscle, which showed less susceptibility to age-related lymphatic decline. The mRNA expression of lymphangiogenic factors was significantly upregulated in SOL and decreased in all muscles of middle-aged rats.

Conclusions

The age-related decrease of lymphatic capillaries in fast-twitch muscles might be associated with age-related muscle atrophy.

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大鼠骨骼肌不同纤维类型的淋巴毛细血管化与生长和年龄有关

方法我们采集了大鼠胫骨前肌（TA）、趾长伸肌（EDL）和比目鱼肌（SOL）。分别使用 LYVE-1 和 CD31 标记对淋巴管和毛细血管进行免疫组化染色。结果肌肉中 LYVE-1 阳性淋巴毛细血管的密度在幼年期达到高峰，随后随着年龄的增长而下降。与血毛细血管不同，快肌优势肌肉（即 TA 和 EDL）的淋巴毛细血管随年龄增长而减少。与血毛细血管类似，淋巴毛细血管在 SOL 肌肉中也很丰富，SOL 肌肉是一种慢速运动优势肌肉，其淋巴衰退与年龄有关的敏感性较低。淋巴管生成因子的 mRNA 表达在 SOL 中显著上调，而在中年大鼠的所有肌肉中均有所下降。

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来源期刊

Microcirculation 医学-外周血管病

CiteScore

5.00

自引率

4.20%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal features original contributions that are the result of investigations contributing significant new information relating to the vascular and lymphatic microcirculation addressed at the intact animal, organ, cellular, or molecular level. Papers describe applications of the methods of physiology, biophysics, bioengineering, genetics, cell biology, biochemistry, and molecular biology to problems in microcirculation. Microcirculation also publishes state-of-the-art reviews that address frontier areas or new advances in technology in the fields of microcirculatory disease and function. Specific areas of interest include: Angiogenesis, growth and remodeling; Transport and exchange of gasses and solutes; Rheology and biorheology; Endothelial cell biology and metabolism; Interactions between endothelium, smooth muscle, parenchymal cells, leukocytes and platelets; Regulation of vasomotor tone; and Microvascular structures, imaging and morphometry. Papers also describe innovations in experimental techniques and instrumentation for studying all aspects of microcirculatory structure and function.