Yuzhen Liu, Christopher D McGann, Mary Krebs, Thomas A Perkins, Rose Fields, Conor K Camplisson, Chris Hsu, Shayan Avanessian, Ashley F Tsue, Evan E Kania, David M Shechner, Brian J Beliveau, Devin K. Schweppe
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引用次数: 0
Abstract
The accuracy of crucial nuclear processes such as transcription, replication, and repair, depends on the local composition of chromatin and the regulatory proteins that reside there. Understanding these DNA-protein interactions at the level of specific genomic loci has remained challenging due to technical limitations. Here, we introduce a method termed "DNA O-MAP", which uses programmable peroxidase-conjugated oligonucleotide probes to biotinylate nearby proteins. We show that DNA O-MAP can be coupled with sample multiplexed quantitative proteomics and next-generation sequencing to quantify DNA-protein and DNA-DNA interactions at specific genomic loci.
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