Ana Caroline de Jesus de Castro, Josiane Celerino de Carvalho, Flávia Camila Schimpl, Joelma Keith Rodrigues, Antônia Vitória Ferreira de Souza, Andreia Varmes Fernandes, Wagner Luíz Araújo, José Francisco de Carvalho Gonçalves
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引用次数: 0
Abstract
Context
A combination of industrial activities, agricultural practices, and poor urban planning has led to soil and plant contamination throughout the world. Heavy metal stress responses in plants have been demonstrated in various studies, but it remains unclear how heavy metals affect tree germination and initial establishment.
Aims
With a focus on species selection for phytoremediation programs in areas with copper (Cu) soil contamination, we aimed to evaluate seed germination, initial growth, and carbohydrate and protein metabolism of seedlings of Hymenaea courbaril, Stryphnodendron pulcherrimum, and Hevea brasiliensis subjected to normal and high (500 mg kg−1) concentrations of Cu. We hypothesised that there are differences in the Cu effects and plant responses on germination, initial growth, and the metabolism of seedlings from different Amazon tree species.
Methods
The germination experiment was conducted with seeds of the tree forest species in the absence or presence of Cu. After germination-parameter analyses, biometric variables, soluble sugars, starch, and proteins were measured.
Key results
In the presence of Cu, the germination traits, and morphological variables of H. brasiliensis were changed, whereas Cu did not strongly affect the other species. All species showed a high metabolic adjustment capacity, with little impact on the metabolism of carbon and nitrogen-related compounds in seeds. Overall, it was therefore possible to verify that S. pulcherrimum and H. courbaril were able to tolerate Cu because of their good performance in the germination stage and maintenance of essential metabolites, whereas H. brasiliensis was more sensitive to the presence of this heavy metal.
Conclusions
Our results provided insights into the ability of tree species to cope with Cu stress and the hypothesis has been confirmed to interspecific differential tolerance.
Implications
Amazonian tree species show valuable potential for improving phytoremediation programs with a focus on soil contamination by Cu.
背景工业活动、农业实践和糟糕的城市规划共同导致了世界各地的土壤和植物污染。植物的重金属应激反应已在多项研究中得到证实，但重金属如何影响树木的发芽和初期生长仍不清楚。目的为了在土壤受铜（Cu）污染的地区选择植物修复计划所需的物种，我们评估了Hymenaea courbaril、Stryphnodendron pulcherrimum和Hevea brasiliensis幼苗在正常和高浓度（500 mg kg-1）Cu条件下的种子萌发、初始生长以及碳水化合物和蛋白质代谢情况。我们假设铜对亚马逊不同树种幼苗的发芽、初始生长和新陈代谢的影响和植物反应存在差异。方法在无铜或有铜的条件下对森林树种的种子进行萌发实验。萌发参数分析后，测量了生物计量变量、可溶性糖、淀粉和蛋白质。主要结果 在有 Cu 的情况下，H. brasiliensis 的发芽性状和形态变量发生了变化，而 Cu 对其他树种的影响不大。所有物种都表现出很高的代谢调节能力，对种子中碳和氮相关化合物的代谢影响很小。因此，总的来说，可以证实 S. pulcherrimum 和 H. courbaril 能够耐受铜，因为它们在萌芽阶段表现良好，并能维持必需代谢物，而 H. brasiliensis 对这种重金属的存在更为敏感。结论我们的研究结果有助于深入了解树种应对铜胁迫的能力，并证实了种间耐受性差异的假设。启示亚马逊树种在改善植物修复计划方面显示出宝贵的潜力，该计划重点关注土壤中的铜污染。
期刊介绍：
Australian Journal of Botany is an international journal for publication of original research in plant science. We seek papers of broad interest with relevance to Southern Hemisphere ecosystems. Our scope encompasses all approaches to understanding plant biology.
Australian Journal of Botany is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.
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