Effect of the Secretome of Mesenchymal Placenta Stem Cells on the Functional Properties of Lewis Lung Carcinoma Cells In Vitro

Pub Date : 2024-07-23 DOI:10.3103/s0095452724040054

L. V. Garmanchuk, G. V. Ostrovska, I. A. Stupak, T. V. Bukreieva, O. I. Gorbach, L. I. Ostapchenko

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Abstract

This paper concerns the effect produced by the components of a conditioned medium (K‑medium), in which mesenchymal human placenta cells (hP-MSC) are cultivated, on the characteristics of Lewis lung carcinoma (LLC) cells in the culture. It is shown for the first time that the K-medium (secretome) components have a prooncogenic effect on LLC cells as evidence by an increase in cell survival rates, LLC cell proliferation stimulation, and a decrease in the level of apoptotic cells. The effect of the K-medium on the adhesion characteristics of LLC cells in the process of their monolayer growth and migration from 3D-cultures is also demonstrated. When the hP-MSC secretome interacts with the cultured LLC cells, the production of proinflammatory cytokines TGF β and Il-6 is observed to grow. At the same time, the proangiogenic factor VEGF remains almost at the same level. Similar changes in the microenvironment during the interaction of mesenchymal and tumor cells may underlie various prooncogenic effects observed in our previous studies with different MSC inoculation methods during the development and metastasis of Lewis lung carcinoma in vivo.

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间充质胎盘干细胞分泌组对体外刘易斯肺癌细胞功能特性的影响

摘要本文涉及培养间充质人胎盘细胞（hP-MSC）的条件培养基（K-medium）的成分对培养中的路易斯肺癌（LLC）细胞特性的影响。研究首次表明，K-medium（分泌组）成分对LLC细胞具有促癌作用，这体现在细胞存活率的提高、LLC细胞增殖的刺激以及凋亡细胞水平的降低上。K介质对LLC细胞在单层生长和从三维培养基迁移过程中的粘附特性的影响也得到了证实。当 hP-MSC 分泌组与培养的 LLC 细胞相互作用时，可观察到促炎细胞因子 TGF β 和 Il-6 的产生量增加。与此同时，促血管生成因子 VEGF 几乎保持在同一水平。间充质干细胞与肿瘤细胞相互作用过程中微环境的类似变化，可能是我们之前用不同的间充质干细胞接种方法在体内观察到的刘易斯肺癌发展和转移过程中各种促癌作用的基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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