Assessment of the legitimacy of cocoa tree hybrids (Theobroma cacao L.) resulting from simple crosses using single nucleotide polymorphism (SNP) markers in Côte d’Ivoire

IF 1.6 3区农林科学 Q2 AGRONOMY

Genetic Resources and Crop Evolution Pub Date : 2024-07-24 DOI:10.1007/s10722-024-02084-2

Okayo Sandrine Minakou, Honorine Brigitte Guiraud, Inago Caudou Trébissou, Klotioloma Coulibaly, Tchréwa Stanislas Kouamé, Mathurin Yves Atchi, Bi Firmin Gouré, Attiapo Pepin Assi, Yaya Ouattara, Sidiky Bakayoko, Gnion Mathias Tahi

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Abstract

The legitimacy of 13 hybrid families resulting from crosses via manual pollination techniques was studied using single nucleotide polymorphism (SNP) markers. The aim of this study was to ensure the conformity of offspring resulting from simple crosses. Fifty-one individuals (39 descendants and 12 parent clones) were analysed using 84 SNP markers. The results revealed nonsignificant genetic differentiation between parents and progenies (P = 0.323). High allelic richness was also revealed, with the average number of alleles per locus ranging from 1.57 to 1.92, with an average of 1.77 in offspring and 2 in parents. Thus, 1% of the total variance was attributed to interpopulation variance, and 99% was attributed to intrapopulation variance. Low genetic differentiation (FST < 0.05) and genetic distances (0.03 < D < 0.09) were observed between hybrid families F2, F8, F10, F11, F12, F13 and F14 and their respective parents. These results confirm the legitimacy of the hybrids produced and suggest that the manual pollination work carried out at CNRA sites is reliable for faithfully reproducing hybrid material using known parents.

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利用单核苷酸多态性（SNP）标记评估科特迪瓦简单杂交产生的可可树杂交种（Theobroma cacao L.）的合法性

利用单核苷酸多态性（SNP）标记对通过人工授粉技术杂交产生的 13 个杂交家系的合法性进行了研究。这项研究的目的是确保简单杂交产生的后代的一致性。使用 84 个 SNP 标记对 51 个个体（39 个后代和 12 个亲本克隆）进行了分析。结果显示，亲本和后代之间的遗传差异不显著（P = 0.323）。等位基因丰富度也很高，每个位点的平均等位基因数从 1.57 到 1.92 不等，子代平均为 1.77，亲代平均为 2。因此，种群间变异占总变异的 1%，种群内变异占总变异的 99%。杂交种 F2、F8、F10、F11、F12、F13 和 F14 与各自亲本之间的遗传分化（FST < 0.05）和遗传距离（0.03 < D < 0.09）较低。这些结果证实了所产生的杂交种的合法性，并表明在中国国家农业研究中心进行的人工授粉工作是可靠的，可以利用已知亲本忠实地繁殖杂交材料。

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来源期刊

Genetic Resources and Crop Evolution 农林科学-农艺学

CiteScore

4.10

自引率

10.00%

发文量

178

审稿时长

3 months

期刊介绍： Genetic Resources and Crop Evolution is devoted to all aspects of plant genetic resources research. It publishes original articles in the fields of taxonomical, morphological, physiological, biochemical, genetical, cytological or ethnobotanical research of genetic resources and includes contributions to gene-bank management in a broad sense, that means to collecting, maintenance, evaluation, storage and documentation. Areas of particular interest include: -crop evolution -domestication -crop-weed relationships -related wild species -history of cultivated plants including palaeoethnobotany. Genetic Resources and Crop Evolution also publishes short communications, e.g. newly described crop taxa, nomenclatural notes, reports of collecting missions, evaluation results of gene-bank material etc. as well as book reviews of important publications in the field of genetic resources. Every volume will contain some review articles on actual problems. The journal is the internationalized continuation of the German periodical Die Kulturpflanze, published formerly by the Institute of Plant Genetics and Crop Plant Research at Gatersleben, Germany. All contributions are in the English language and are subject to peer reviewing.