Multi-Species Probiotics Ameliorate Salinity-Induced Growth Retardation In Striped Catfish Pangasianodon hypophthalmus

IF 2.6 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES
Md Meftahul Zannat, Md Fazle Rohani, Re-One Zannat Jeba, Md Shahjahan
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引用次数: 0

Abstract

Salinity is regarded as one of the most crucial environmental parameters and salinity mediated stress adversely affect the aquaculture production. Probiotics might alleviate the salinity stress in fish. Here, we investigated the role of probiotics on growth (weight gain, specific growth rate), survivability, feed utility (feed conversion ratio), hemoglobin, glucose, structure of erythrocytes (cellular and nuclear abnormalities), and gut morphology of striped catfish (Pangasianodon hypophthalmus) reared in different salinities. The fingerlings (8.10 ± 0.20 g) were evenly distributed four different salinities (0, 4, 8 and 12 ppt) in three replicates with and without probiotics supplementation for 56 days. Multi-species probiotics containing Bacillus subtilis (5 × 109 cfu/ml), B. thuringiencis (4 × 109 cfu/ml), Lactobacillus plantarum (5.8 × 109 cfu/ml), and L. buchneri (6.5 × 109 cfu/ml) were used as 1.0 mL/L at the alternate day. The results of this study indicated that survivability, growth and feed utility were negatively affected with the increased salinity, however multi-species probiotics significantly ameliorated the negative effects of salinity. Moreover, multi-species probiotics significantly improved hemoglobin (g/dL) and glucose (mg/dL) level at different salinity in comparison with the non-probiotics treated groups. Frequencies of cellular and nuclear abnormalities of erythrocytes were increased significantly with the increase of salinity in non-probiotics groups, while those significantly declined in multi-species probiotics treated groups. Increased salinity level suppressed the immune response indices, while probiotics supplementation significantly improved the gut immune response irrespective of salinity level. Taken altogether, the current study revealed that application of multi-species probiotics can be an effective tool to reduce salinity stressors and improve growth and health status by upgrading hemato-biochemical profile and intestinal morphology of P. hypophthalmus at higher salinity.

Abstract Image

多物种益生菌改善盐度引起的带鱼生长迟缓问题
盐度被认为是最重要的环境参数之一,盐度引起的应激对水产养殖生产产生了不利影响。益生菌可以缓解鱼类的盐度应激。在此,我们研究了益生菌对在不同盐度条件下饲养的带鱼(Pangasianodon hypophthalmus)的生长(增重、特定生长率)、存活率、饲料利用率(饲料转化率)、血红蛋白、葡萄糖、红细胞结构(细胞和核异常)以及肠道形态的作用。将幼鱼(8.10 ± 0.20 克)均匀分布在四个不同盐度(0、4、8 和 12 ppt)的三个重复中,分别添加和不添加益生菌,饲养 56 天。隔天使用含枯草芽孢杆菌(5×109 cfu/ml)、苏云金杆菌(4×109 cfu/ml)、植物乳杆菌(5.8×109 cfu/ml)和布氏乳杆菌(6.5×109 cfu/ml)的多菌种益生菌,浓度为 1.0 mL/L。研究结果表明,盐度升高会对存活率、生长和饲料利用率产生负面影响,但多物种益生菌能显著改善盐度的负面影响。此外,与未添加益生菌的组别相比,多菌种益生菌能明显改善不同盐度下的血红蛋白(克/分升)和血糖(毫克/分升)水平。随着盐度的增加,非益生菌组红细胞的细胞和核异常频率明显增加,而多物种益生菌处理组则明显下降。盐度升高抑制了免疫反应指数,而无论盐度高低,补充益生菌都能明显改善肠道免疫反应。综上所述,本研究表明,在较高盐度条件下,应用多物种益生菌可以有效降低盐度胁迫,改善鳙鱼的血液生化指标和肠道形态,从而改善其生长和健康状况。
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来源期刊
CiteScore
5.40
自引率
0.00%
发文量
104
审稿时长
1.7 months
期刊介绍: International Journal of Environmental Research is a multidisciplinary journal concerned with all aspects of environment. In pursuit of these, environmentalist disciplines are invited to contribute their knowledge and experience. International Journal of Environmental Research publishes original research papers, research notes and reviews across the broad field of environment. These include but are not limited to environmental science, environmental engineering, environmental management and planning and environmental design, urban and regional landscape design and natural disaster management. Thus high quality research papers or reviews dealing with any aspect of environment are welcomed. Papers may be theoretical, interpretative or experimental.
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