Consolidation and cytotoxicity analysis of a purification strategy for biotechnological xylitol production using fixed bed column adsorption and nanofiltration membranes

IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Danielle Garcia Ribeiro Galvão , Éderson Paulo Xavier Guilherme , Gabriela de Matuoka e Chiocchetti , Juliana Alves Macedo , Marcus Bruno Soares Forte
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Abstract

Agro-industrial waste is increasingly utilized in biotechnological processes to convert lignocellulosic materials into high-value products, such as xylitol. This polyol can be produced using biotechnological methods that mitigate environmental impacts, but it entails high purification costs. The article proposes a comparative study between two sequential strategies for purifying biotechnological xylitol. The first strategy involves membrane filtration followed by column adsorption. While the second strategy only covers column adsorption with twice the adsorbent bed. Additionally, the study includes a cytotoxicity evaluation of various purified xylitol fractions. Column adsorption was conducted at 70 °C with a flow rate of 1.2 mL min−1 using activated carbon as the adsorbent. It proved to be efficient in separating colored compounds, proteins, and ethanol, with retention coefficients of 99.23 %, 84.0 %, and 96.71 %, respectively. The purification factor of xylitol/ethanol was 14.84. Nanofiltration was performed using a poly (piperazine amide) membrane at 40 °C and 30 bar, resulting in a protein retention of 43.55 % and a xylitol purity of 27.73 %. Finally, purified xylitol fractions underwent cytotoxicity analysis using the MTT assay, conducted in intestinal epithelial cells (Caco-2). One of the analyzed fractions did not induce toxicity, demonstrating that activated carbon column adsorption was the most effective strategy for purifying biotechnologically produced xylitol. These findings contribute to enhancing the viability of biotechnological xylitol production from sugarcane bagasse hemicellulosic hydrolysate.

Abstract Image

利用固定床柱吸附和纳滤膜生产木糖醇的生物技术纯化策略的巩固和细胞毒性分析
生物技术工艺越来越多地利用农用工业废料将木质纤维素材料转化为高价值产品,如木糖醇。这种多元醇可通过生物技术方法生产,从而减轻对环境的影响,但提纯成本较高。文章提出了对生物技术木糖醇提纯的两种顺序策略进行比较研究。第一种策略涉及膜过滤,然后是柱吸附。而第二种策略只包括两倍吸附剂床的柱吸附。此外,该研究还包括对各种纯化木糖醇馏分的细胞毒性评估。柱吸附以活性炭为吸附剂,在 70 °C 下以 1.2mLmin 的流速进行。结果表明,该方法能有效分离有色化合物、蛋白质和乙醇,保留系数分别为 99.23%、84.0% 和 96.71%。木糖醇/乙醇的纯化系数为 14.84。在 40 °C 和 30bar 条件下使用聚(哌嗪酰胺)膜进行纳滤,蛋白质保留率为 43.55%,木糖醇纯度为 27.73%。最后,纯化的木糖醇馏分在肠上皮细胞(Caco-2)中使用 MTT 试验进行细胞毒性分析。其中一个分析馏分没有引起毒性,这表明活性炭柱吸附是纯化生物技术生产的木糖醇的最有效策略。这些发现有助于提高利用甘蔗渣半纤维素水解物生产木糖醇的生物技术可行性。
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来源期刊
Food and Bioproducts Processing
Food and Bioproducts Processing 工程技术-工程:化工
CiteScore
9.70
自引率
4.30%
发文量
115
审稿时长
24 days
期刊介绍: Official Journal of the European Federation of Chemical Engineering: Part C FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering. Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing. The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those: • Primarily concerned with food formulation • That use experimental design techniques to obtain response surfaces but gain little insight from them • That are empirical and ignore established mechanistic models, e.g., empirical drying curves • That are primarily concerned about sensory evaluation and colour • Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material, • Containing only chemical analyses of biological materials.
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