Co-existence of two plasmids harboring transferable resistance-nodulation-division pump gene cluster, tmexCD1-toprJ1, and colistin resistance gene mcr-8 in Klebsiella pneumoniae.

IF 4.6 2区 医学 Q1 MICROBIOLOGY
Xiaofen Mo, Hui Zhang, Junfeng Fan, Linna Xu, Hao Fu, Junpeng Yue, Kaixuan Dong, Qixia Luo, Fen Wan
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引用次数: 0

Abstract

Background: The emergence of plasmid-mediated mobile colistin resistance (mcr) gene poses a great challenge to the clinical application of polymyxins. To date, mcr-1 to mcr-10 have been found in animals, humans, and the environment. Among them, mcr-8 was first identified in Klebsiella pneumoniae (K. pneumoniae) of swine origin, and then mcr-8.1 to mcr-8.5 were successively identified. Notably, K. pneumoniae is the major host of the mcr-8 gene in both animals and humans. This study aims to explore the characteristics of K. pneumoniae strains carrying the mcr-8 gene and tmexCD1-toprJ1 gene cluster and investigate the correlation between these two antibiotic resistance genes.

Methods: The isolates from the poultry farms and the surrounding villages were identified by mass spectrometer, and the strains positive for mcr-1 to mcr-10 were screened by polymerase chain reaction (PCR). The size of the plasmid and the antimicrobial resistance genes carried were confirmed by S1-nuclease pulsed-field gel electrophoresis (S1-PFGE) and Southern hybridization, and the transferability of the plasmid was verified by conjugation experiments. Antimicrobial susceptibility testing (AST) and whole genome sequencing (WGS) were used to characterize the strains.

Results: Two K. pneumoniae isolates (KP26 and KP29) displaying polymyxin resistance were identified as mcr-8 gene carriers. Besides that, tigecycline-resistant gene cluster tmexCD1-toprJ1 was also found on the other plasmid which conferred strain resistance to tigecycline. Through epidemiological analysis, we found that the mcr-8 gene has dispersed globally, circulating in the human, animals, and the environment. Furthermore, our analysis suggests that the coexistence of mcr-8 and tmexCD1-toprJ1 on a single plasmid might evolved through plasmid recombination.

Conclusions: Although the mcr-8 and tmexCD1-toprJ1 gene clusters in the two strains of K. pneumoniae in this study were on two different plasmids, they still pose a potential threat to public health, requiring close monitoring and further study.

肺炎克雷伯氏菌中携带可转移抗性-结节-分裂泵基因簇 tmexCD1-toprJ1 和可乐定抗性基因 mcr-8 的两种质粒共存。
背景:质粒介导的可乐定耐药性(mcr)基因的出现给多粘菌素的临床应用带来了巨大挑战。迄今为止,已在动物、人类和环境中发现了 mcr-1 至 mcr-10。其中,mcr-8 最早在猪源肺炎克雷伯菌(K. pneumoniae)中被发现,随后 mcr-8.1 至 mcr-8.5 相继被发现。值得注意的是,肺炎克雷伯菌是 mcr-8 基因在动物和人类中的主要宿主。本研究旨在探讨携带 mcr-8 基因和 tmexCD1-toprJ1 基因簇的肺炎克氏菌菌株的特征,并研究这两种抗生素耐药基因之间的相关性:方法:用质谱仪鉴定从养鸡场和周边村庄分离的菌株,用聚合酶链反应(PCR)筛选 mcr-1 至 mcr-10 阳性菌株。通过 S1 核酸酶脉冲场凝胶电泳(S1-PFGE)和 Southern 杂交确认了质粒的大小和携带的抗菌药耐药基因,并通过共轭实验验证了质粒的可转移性。抗菌药物敏感性测试(AST)和全基因组测序(WGS)用于鉴定菌株的特征:结果:两株对多粘菌素具有耐药性的肺炎双球菌(KP26 和 KP29)被鉴定为 mcr-8 基因携带者。此外,在另一个质粒上也发现了耐替加环素基因簇 tmexCD1-toprJ1,该基因簇赋予菌株对替加环素的耐药性。通过流行病学分析,我们发现 mcr-8 基因已在全球扩散,在人类、动物和环境中循环。此外,我们的分析表明,mcr-8 和 tmexCD1-toprJ1 共存于一个质粒上可能是通过质粒重组进化而来的:结论:尽管本研究中两株肺炎克氏菌的 mcr-8 和 tmexCD1-toprJ1 基因簇位于两个不同的质粒上,但它们仍对公共卫生构成潜在威胁,需要密切监测和进一步研究。
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来源期刊
CiteScore
8.60
自引率
0.00%
发文量
49
审稿时长
>12 weeks
期刊介绍: Annals of Clinical Microbiology and Antimicrobials considers good quality, novel and international research of more than regional relevance. Research must include epidemiological and/or clinical information about isolates, and the journal covers the clinical microbiology of bacteria, viruses and fungi, as well as antimicrobial treatment of infectious diseases. Annals of Clinical Microbiology and Antimicrobials is an open access, peer-reviewed journal focusing on information concerning clinical microbiology, infectious diseases and antimicrobials. The management of infectious disease is dependent on correct diagnosis and appropriate antimicrobial treatment, and with this in mind, the journal aims to improve the communication between laboratory and clinical science in the field of clinical microbiology and antimicrobial treatment. Furthermore, the journal has no restrictions on space or access; this ensures that the journal can reach the widest possible audience.
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