Identification and characterization of Staphylococcus argenteus from Indonesia

IF 4.5 3区 医学 Q1 MICROBIOLOGY
Indri Rooslamiati Supriadi , Dewi Santosaningsih , Nyoman S. Budayanti , Willemien H.A. Zandijk , Amber Rijfkogel , Corné H.W. Klaassen , Juliëtte A. Severin
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Abstract

Background

In 2015, Staphylococcus argenteus was reported for the first time as a novel species of the Staphylococcus aureus complex. While S. argenteus has been found in many countries, its presence in Indonesia has not been reported yet. Our aim is to confirm S. argenteus presence in Indonesia, describe its characteristics and analyze its genomic diversity.

Methods

The S. aureus isolates used in this study were collected from patients with skin and soft tissue infections in Indonesia, between July 2009 to February 2010. Randomly selected isolates were recultured from −80 C° stocks and analyzed using matrix-assisted laser desorption/ionization – time of flight (MALDI-TOF). Isolates identified as S. argenteus, S. roterodami, or S. schweitzeri and S. aureus with a low score in the MALDI-TOF analysis were analyzed by a real-time PCR targeting the nucA gene able to identify true S. argenteus. Isolates identified as S. argenteus were further characterized by whole genome sequencing. Vitek®2 (bioMérieux) was used for antimicrobial susceptibility testing.

Results

Fifteen isolates were identified as S. argenteus, with the majority belonging to ST2250. Two pairs of isolates proved to be identical by core genome multilocus sequence typing analysis. Most isolates were susceptible to all antibiotics tested, except for seven isolates (46.7 %) that were resistant to benzylpenicillin, and one isolate was resistant to tetracycline (6.7 %). The presence of resistance genes blaZ and tet(45) correlated with these findings. Notably, the sey enterotoxin gene was prevalent in 80 % of the isolates. Other virulence factor genes were less prevalent. Plasmid replicon types in S. argenteus were also known to S. aureus.

Conclusion

Our study reveals the occurrence of S. argenteus in Indonesia. The diversity within Indonesian S. argenteus matches the global diversity of S. argenteus. Identical isolates between patients indicate potential transmission events. A lower prevalence of a broad panel of virulence factors suggests that S. argenteus is less virulent than S. aureus.

印度尼西亚阿根廷葡萄球菌的鉴定和特征描述。
背景:2015 年,首次报道了金黄色葡萄球菌(Staphylococcus argenteus)是金黄色葡萄球菌复合体中的一个新物种。虽然在许多国家都发现了 S. argenteus,但印尼尚未报道其存在。我们的目的是确认 S. argenteus 在印尼的存在,描述其特征并分析其基因组多样性:本研究中使用的金黄色葡萄球菌分离物来自 2009 年 7 月至 2010 年 2 月期间印度尼西亚的皮肤和软组织感染患者。随机选取的分离物从-80 C°的存货中重新培养,并使用基质辅助激光解吸/电离-飞行时间(MALDI-TOF)进行分析。在 MALDI-TOF 分析中得分较低的被鉴定为 S. argenteus、S. roterodami 或 S. schweitzeri 和 S. aureus 的分离物,则通过针对 nucA 基因的实时 PCR 进行分析,以鉴定真正的 S. argenteus。被鉴定为 S. argenteus 的分离物通过全基因组测序进行了进一步鉴定。Vitek®2 (bioMérieux) 用于抗菌药敏感性测试:结果:15 个分离株被鉴定为 S. argenteus,其中大多数属于 ST2250。通过核心基因组多焦点序列分型分析,有两对分离物被证明是相同的。除了 7 个分离株(46.7%)对苄青霉素耐药、1 个分离株对四环素耐药(6.7%)外,大多数分离株对所有测试的抗生素都敏感。耐药基因 blaZ 和 tet(45) 的存在与这些发现相关。值得注意的是,80% 的分离株中普遍存在 sey 肠毒素基因。其他毒力因子基因的流行率较低。阿根廷痢疾杆菌的质粒复制子类型与金黄色葡萄球菌相同:我们的研究揭示了 S. argenteus 在印度尼西亚的存在。结论:我们的研究揭示了银环蛇属细菌在印尼的存在,印尼银环蛇属细菌的多样性与全球银环蛇属细菌的多样性相吻合。患者之间的相同分离物表明可能存在传播事件。各种毒力因子的流行率较低,这表明阿根廷痢疾杆菌的毒力低于金黄色葡萄球菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
9.70
自引率
0.00%
发文量
18
审稿时长
45 days
期刊介绍: Pathogen genome sequencing projects have provided a wealth of data that need to be set in context to pathogenicity and the outcome of infections. In addition, the interplay between a pathogen and its host cell has become increasingly important to understand and interfere with diseases caused by microbial pathogens. IJMM meets these needs by focussing on genome and proteome analyses, studies dealing with the molecular mechanisms of pathogenicity and the evolution of pathogenic agents, the interactions between pathogens and host cells ("cellular microbiology"), and molecular epidemiology. To help the reader keeping up with the rapidly evolving new findings in the field of medical microbiology, IJMM publishes original articles, case studies and topical, state-of-the-art mini-reviews in a well balanced fashion. All articles are strictly peer-reviewed. Important topics are reinforced by 2 special issues per year dedicated to a particular theme. Finally, at irregular intervals, current opinions on recent or future developments in medical microbiology are presented in an editorial section.
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