Just passing through: Deploying aquaporins in microbial cell factories.

IF 2.5 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Liam Richard Jenkins Sánchez, Lobke Maria Sips, Inge Noëlle Adriënne Van Bogaert
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引用次数: 0

Abstract

As microbial membranes are naturally impermeable to even the smallest biomolecules, transporter proteins are physiologically essential for normal cell functioning. This makes transporters a key target area for engineering enhanced cell factories. As part of the wider cellular transportome, aquaporins (AQPs) are responsible for transporting small polar solutes, encompassing many compounds which are of great interest for industrial biotechnology, including cell feedstocks, numerous commercially relevant polyols and even weak organic acids. In this review, examples of cell factory engineering by targeting AQPs are presented. These AQP modifications aid in redirecting carbon fluxes and boosting bioconversions either by enhanced feedstock uptake, improved intermediate retention, increasing product export into the media or superior cell viability against stressors with applications in both bacterial and yeast production platforms. Additionally, the future potential for AQP deployment and targeting is discussed, showcasing hurdles and considerations of this strategy as well as recent advances and future directions in the field. By leveraging the natural diversity of AQPs and breakthroughs in channel protein engineering, these transporters are poised to be promising tools capable of enhancing a wide variety of biotechnological processes.

只是经过:在微生物细胞工厂中部署水汽蛋白
由于微生物膜天生无法渗透最小的生物分子,因此转运蛋白在生理上对细胞的正常运作至关重要。这使得转运体成为工程强化细胞工厂的关键目标领域。作为更广泛的细胞转运体的一部分,水蒸发蛋白(AQPs)负责转运小型极性溶质,其中包括许多对工业生物技术具有重大意义的化合物,包括细胞原料、许多具有商业价值的多元醇甚至弱有机酸。本综述介绍了以 AQPs 为目标的细胞工厂工程实例。这些 AQP 改造有助于重新定向碳通量和促进生物转化,具体方法包括增强原料吸收、改善中间体保留、增加产品向培养基的输出或提高细胞对应激源的存活能力,可应用于细菌和酵母生产平台。此外,还讨论了 AQP 部署和靶向的未来潜力,展示了这一战略的障碍和注意事项,以及该领域的最新进展和未来方向。通过利用 AQPs 的天然多样性和通道蛋白工程学的突破,这些转运体有望成为增强各种生物技术过程的有前途的工具。
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来源期刊
Biotechnology Progress
Biotechnology Progress 工程技术-生物工程与应用微生物
CiteScore
6.50
自引率
3.40%
发文量
83
审稿时长
4 months
期刊介绍: Biotechnology Progress , an official, bimonthly publication of the American Institute of Chemical Engineers and its technological community, the Society for Biological Engineering, features peer-reviewed research articles, reviews, and descriptions of emerging techniques for the development and design of new processes, products, and devices for the biotechnology, biopharmaceutical and bioprocess industries. Widespread interest includes application of biological and engineering principles in fields such as applied cellular physiology and metabolic engineering, biocatalysis and bioreactor design, bioseparations and downstream processing, cell culture and tissue engineering, biosensors and process control, bioinformatics and systems biology, biomaterials and artificial organs, stem cell biology and genetics, and plant biology and food science. Manuscripts concerning the design of related processes, products, or devices are also encouraged. Four types of manuscripts are printed in the Journal: Research Papers, Topical or Review Papers, Letters to the Editor, and R & D Notes.
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