Enhanced production of isobutyl and isoamyl acetate using Yarrowia lipolytica.

IF 2.5 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Ayumi Koshiba, Mariko Nakano, Yuuki Hirata, Rie Konishi, Yuta Matsuoka, Yuta Miwa, Ayana Mori, Akihiko Kondo, Tsutomu Tanaka
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引用次数: 0

Abstract

Short-chain esters, particularly isobutyl acetate and isoamyl acetate, hold significant industrial value due to their wide-ranging applications in flavors, fragrances, solvents, and biofuels. In this study, we demonstrated the biosynthesis of acetate esters using Yarrowia lipolytica as a host by feeding alcohols to the yeast culture. Initially, we screened for optimal alcohol acyltransferases for ester biosynthesis in Y. lipolytica. Strains of Y. lipolytica expressing atf1 from Saccharomyces cerevisiae, produced 251 or 613 mg/L of isobutyl acetate or of isoamyl acetate, respectively. We found that introducing additional copies of ATF1 enhanced ester production. Furthermore, by increasing the supply of acetyl-CoA and refining the culture conditions, we achieved high production of isoamyl acetate, reaching titers of 3404 mg/L. We expanded our study to include the synthesis of a range of acetate esters, facilitated by enriching the culture medium with various alcohols. This study underscores the versatility and potential of Y. lipolytica in the industrial production of acetate esters.

利用脂肪分解蓍草菌提高乙酸异丁酯和乙酸异戊酯的产量。
短链酯类,尤其是醋酸异丁酯和醋酸异戊酯,因其在香精、香料、溶剂和生物燃料方面的广泛应用而具有重要的工业价值。在本研究中,我们以脂肪分解酵母菌为宿主,通过向酵母培养物中添加酒精,证明了醋酸酯的生物合成。首先,我们筛选了脂肪溶解酵母中用于酯类生物合成的最佳醇酰基转移酶。表达来自酿酒酵母的 atf1 的溶脂酵母菌株分别产生了 251 或 613 mg/L 的乙酸异丁酯或乙酸异戊酯。我们发现,引入额外拷贝的 ATF1 可提高酯的产量。此外,通过增加乙酰-CoA 的供应量和改进培养条件,我们获得了较高的乙酸异戊酯产量,滴度达到 3404 毫克/升。我们将研究范围扩大到了一系列乙酸酯的合成,并通过在培养基中添加各种醇类来促进合成。这项研究强调了脂肪溶解酵母在工业化生产醋酸酯方面的多功能性和潜力。
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来源期刊
Biotechnology Progress
Biotechnology Progress 工程技术-生物工程与应用微生物
CiteScore
6.50
自引率
3.40%
发文量
83
审稿时长
4 months
期刊介绍: Biotechnology Progress , an official, bimonthly publication of the American Institute of Chemical Engineers and its technological community, the Society for Biological Engineering, features peer-reviewed research articles, reviews, and descriptions of emerging techniques for the development and design of new processes, products, and devices for the biotechnology, biopharmaceutical and bioprocess industries. Widespread interest includes application of biological and engineering principles in fields such as applied cellular physiology and metabolic engineering, biocatalysis and bioreactor design, bioseparations and downstream processing, cell culture and tissue engineering, biosensors and process control, bioinformatics and systems biology, biomaterials and artificial organs, stem cell biology and genetics, and plant biology and food science. Manuscripts concerning the design of related processes, products, or devices are also encouraged. Four types of manuscripts are printed in the Journal: Research Papers, Topical or Review Papers, Letters to the Editor, and R & D Notes.
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