Specialization of the Stems into Shoots, Stolons, and Rhizomes in Bermudagrass (Cynodon dactylon L.): Insights from Combined Metabolome and Transcriptome Analyses
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引用次数: 0
Abstract
As a perennial warm-season turfgrass species with great economic value, bermudagrass (Cynodon dactylon L.) simultaneously has three types of stems: shoot, stolon, and rhizome. However, molecular mechanisms underlying the specialization of the three types of stems remain poorly understood. In this study, the metabolome differences among the three types of stems were analyzed and compared through untargeted metabolomic profiling in combination with transcriptome-wide analyses of the genes participating in the metabolic pathways. A total of 949 metabolites were identified in the three stems, whereas 303, 473, and 330 metabolites were differentially accumulated between shoots and stolons, shoots and rhizomes, and stolons and rhizomes, respectively. Sugars and phenylpropanoids were two enriched categories of metabolites showing preferential accumulation in the three types of stems. Transcriptome and RT-qPCR analyses indicated that gene expression of key enzymes catalyzing the synthesis and transformation of sugars and phenylpropanoids, especially glucose-1-phosphate adenylyltransferase, starch synthase, and phenylalanine ammonia-lyase, were delicately regulated to maintain the sugar-starch and lignin-flavonoid homeostasis in the three stems. The results of this study not only expanded our understanding of metabolism regulation in bermudagrass, but also laid a foundation for molecular mechanism study of stem specialization in this glamorous plant species.
期刊介绍:
The Journal of Plant Growth Regulation is an international publication featuring original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research on various aspects of plant growth and development using hormonal, physiological, environmental, genetic, biophysical, developmental and/or molecular approaches.
The journal also publishes timely reviews on highly relevant areas and/or studies in plant growth and development, including interdisciplinary work with an emphasis on plant growth, plant hormones and plant pathology or abiotic stress.
In addition, the journal features occasional thematic issues with special guest editors, as well as brief communications describing novel techniques and meeting reports.
The journal is unlikely to accept manuscripts that are purely descriptive in nature or reports work with simple tissue culture without attempting to investigate the underlying mechanisms of plant growth regulation, those that focus exclusively on microbial communities, or deal with the (elicitation by plant hormones of) synthesis of secondary metabolites.