Exploring lipase-based biocatalysts derived from Yarrowia lipolytica for fatty acid ethyl esters synthesis

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-19 DOI:10.1007/s43153-024-00492-6

Jéssica R. da Silva, Kelly C. N. R. Pedro, Gabrielle A. R. da Silva, Aline M. Castro, Maria Alice Z. Coelho, Bernardo D. Ribeiro, Cristiane A. Henriques, Marta A. P. Langone

{"title":"Exploring lipase-based biocatalysts derived from Yarrowia lipolytica for fatty acid ethyl esters synthesis","authors":"Jéssica R. da Silva, Kelly C. N. R. Pedro, Gabrielle A. R. da Silva, Aline M. Castro, Maria Alice Z. Coelho, Bernardo D. Ribeiro, Cristiane A. Henriques, Marta A. P. Langone","doi":"10.1007/s43153-024-00492-6","DOIUrl":null,"url":null,"abstract":"This work aimed to evaluate the lipases from Yarrowia lipolytica as a biocatalyst for synthesizing fatty acid ethyl esters (FAEE). The Yarrowia lipolytica lipases were evaluated in three distinct forms: firstly, as the crude enzymatic liquid extract derived from the submerged fermentation process of Y. lipolytica (YLL); secondly, as YLL immobilized on silica-alumina (YLL-S40); and thirdly, as the fermented solid material obtained from solid-state fermentation of Y. lipolytica (SEP-YLL). The highest FAEE content (54 wt.%) was obtained after 240 h in transesterification reactions using SEP-YLL. The influence of temperature, biocatalyst concentration, and reaction time was studied in the ethyl oleate synthesis. The SEP-YLL also showed better results compared to YLL and YLL-S40. The reaction catalyzed by SEP-YLL achieved 81% conversion at 30 °C. The SEP-YLL was evaluated in a batch-stirred reactor (BSTR) and a continuous packed-bed reactor (PBR), obtaining an oleic acid conversion of 80% and 70%, respectively. The low-cost biocatalyst (SEP-YLL) demonstrated potential for application in biodiesel production by reducing free fatty acids from an acid oil source through PBR and BSTR.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s43153-024-00492-6","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

This work aimed to evaluate the lipases from Yarrowia lipolytica as a biocatalyst for synthesizing fatty acid ethyl esters (FAEE). The Yarrowia lipolytica lipases were evaluated in three distinct forms: firstly, as the crude enzymatic liquid extract derived from the submerged fermentation process of Y. lipolytica (YLL); secondly, as YLL immobilized on silica-alumina (YLL-S40); and thirdly, as the fermented solid material obtained from solid-state fermentation of Y. lipolytica (SEP-YLL). The highest FAEE content (54 wt.%) was obtained after 240 h in transesterification reactions using SEP-YLL. The influence of temperature, biocatalyst concentration, and reaction time was studied in the ethyl oleate synthesis. The SEP-YLL also showed better results compared to YLL and YLL-S40. The reaction catalyzed by SEP-YLL achieved 81% conversion at 30 °C. The SEP-YLL was evaluated in a batch-stirred reactor (BSTR) and a continuous packed-bed reactor (PBR), obtaining an oleic acid conversion of 80% and 70%, respectively. The low-cost biocatalyst (SEP-YLL) demonstrated potential for application in biodiesel production by reducing free fatty acids from an acid oil source through PBR and BSTR.

Abstract Image

查看原文本刊更多论文

探索从脂肪分解亚罗菌中提取的基于脂肪酶的脂肪酸乙酯合成生物催化剂

这项研究旨在评估脂肪分解亚罗威氏菌（Yarrowia lipolytica）的脂肪酶作为合成脂肪酸乙酯（FAEE）的生物催化剂的效果。对三种不同形式的脂肪分解酵母脂肪酶进行了评估：第一种是从脂肪分解酵母浸没发酵过程中提取的粗酶液提取物（YLL）；第二种是固定在二氧化硅-氧化铝上的 YLL（YLL-S40）；第三种是脂肪分解酵母固态发酵得到的发酵固体材料（SEP-YLL）。使用 SEP-YLL 进行酯交换反应 240 小时后，FAEE 含量最高（54 wt.%）。研究了温度、生物催化剂浓度和反应时间对油酸乙酯合成的影响。与 YLL 和 YLL-S40 相比，SEP-YLL 也显示出更好的效果。SEP-YLL 催化的反应在 30 °C 时的转化率达到 81%。在间歇搅拌反应器（BSTR）和连续填料床反应器（PBR）中对 SEP-YLL 进行了评估，油酸转化率分别为 80% 和 70%。低成本生物催化剂（SEP-YLL）通过 PBR 和 BSTR 还原酸性油源中的游离脂肪酸，展示了在生物柴油生产中的应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.