MELATONIN INDUCES CHANGES ON THE CIRCADIAN RHYTHMS OF REPRODUCTIVE HORMONES DURING SPERMATOGENESIS IN PUBERTAL MALE SEA BASS, Dicentrarchus labrax

Maria Victoria Alvarado, Felipe Espigares, Manuel Carrillo, Alicia Felip
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Abstract

Reproduction is a highly demanding biological process that occurs at the optimal time of the year and day to ensure the success of spawn and offspring. Melatonin is a hormone that, secreted mainly by the pineal gland, plays a critical role in the integration of the photoneuroendocrine information from environment (annual and daily variations) to modulate reproductive activity and gonadal development in fish. In this study we assessed the effect of exogenous melatonin on the circadian levels of sex steroids and gonadotropins in pubertal 2 yr-old male sea bass during their reproductive cycle including, pre-spermatogenesis (Pspg), spermiation (Spm) and post-spermiation (PSpm) stages. Our results demonstrated that all reproductive hormones displayed circadian variations along the entire reproductive cycle in pubertal fish. Circulating levels of the luteinizing hormone (Lh) were affected by both melatonin injection and the daily timing of administration during the Spm and PSpm stages, thus evoking variations of Lh levels at night. Melatonin also significantly affected circadian rhythms of Fsh during the Spm stage. Overall, both 11-Kt and T plasma levels displayed circadian variations during the reproductive cycle in the sea bass which were not prevented by melatonin. However, melatonin showed a significant decrease of plasma levels of 11-Kt 1h after dusk during the Pspg stage, while it increased those levels of T 5 h after dusk during the PSpm stage. These findings provide new insights into the role of melatonin in fish reproduction as a key factor in regulation of daily variation of key hormones involved in gonadal development. This circumstance may have implications in the control of gametogenesis and management of fish in aquaculture.
梅拉通宁诱导生殖雌性海鲈(Dicentrarchus labrax)在精子发生过程中生殖激素循环节律的变化
繁殖是一个要求很高的生物过程,需要在一年中的最佳时间和一天中的最佳时间进行,以确保产卵和后代的成功。褪黑素是一种激素,主要由松果体分泌,在整合来自环境的光神经内分泌信息(年变化和日变化)以调节鱼类的生殖活动和性腺发育方面发挥着关键作用。在这项研究中,我们评估了外源性褪黑激素对青春期两岁雄性海鲈生殖周期(包括精子发生前期(Pspg)、精子发生期(Spm)和精子发生后期(PSpm))中性激素和促性腺激素昼夜节律水平的影响。我们的研究结果表明,在青春期鱼类的整个生殖周期中,所有生殖激素都显示出昼夜节律变化。在Spm和PSpm阶段,黄体生成素(Lh)的循环水平受到褪黑激素注射和每日给药时间的影响,从而引起夜间Lh水平的变化。在Spm阶段,褪黑激素也会明显影响Fsh的昼夜节律。总体而言,11-Kt和T的血浆水平在海鲈的生殖周期中都显示出昼夜节律变化,但褪黑激素并不能阻止这种变化。然而,褪黑激素能显著降低Pspg阶段黄昏后1小时的11-Kt血浆水平,而提高PSpm阶段黄昏后5小时的T血浆水平。这些发现为褪黑激素在鱼类繁殖中的作用提供了新的见解,因为褪黑激素是调节性腺发育关键激素日变化的一个关键因素。这种情况可能会对配子发生的控制和水产养殖中的鱼类管理产生影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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