Mechanism of Stellate Ganglion Block Anesthesia Inhibiting Neuronal Apoptosis in Rats by Down-regulating Bax gene

IF 1.9 4区 计算机科学 Q3 TELECOMMUNICATIONS
Qiao Xu, Lei Li, Ke Yan, Siqi Ye
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Abstract

To explore the feasibility of stellate ganglion block (SGB) for the treatment of brain injury and the inhibitory effect of neuronal apoptosis. SD rats were randomly divided into control group, model group (LPS 0.6 mg/kg), and SGB group (LPS 0.6 mg/kg + lidocaine 0.2 mL). The rats were sacrificed after SGB block for 2 h to separate the hippocampus. The pathological changes of hippocampal tissue were observed by HE staining, the apoptosis of hippocampal neurons was observed by TUNEL staining, the positive expression of B-cell lymphoma-2-Associated X (Bax) in hippocampal tissue was detected by immunohistochemistry, and the expression of Bax mRNA in hippocampal tissue was detected by qRT-PCR method. Results: Compared with the control group, the hippocampal tissue of the model group was severely damaged, and the apoptosis rate of hippocampal neurons, the positive rate of Bax protein, the expression of Bax mRNA, and the protein expressions of Baxwere significantly increased, the protein expression of Bcl-2 was significantly decreased (P < 0.05).Compared with the model group, the hippocampal tissue damage of the SGB group was significantly reduced, and the hippocampal neuron apoptosis rate, Bax protein positive rate, Bax mRNA expression and Bax protein expressions were significantly decreased, and the expression of Bcl-2 protein was significantly increased (P < 0.05). Conclusion: SGB can reduce the apoptosis of neurons in rats and improve brain injury by inhibiting the expression of Bax gene.

Abstract Image

星状神经节阻滞麻醉通过下调 Bax 基因抑制大鼠神经元凋亡的机制
探讨星状神经节阻滞(SGB)治疗脑损伤的可行性以及对神经细胞凋亡的抑制作用。将 SD 大鼠随机分为对照组、模型组(LPS 0.6 mg/kg)和 SGB 组(LPS 0.6 mg/kg + 利多卡因 0.2 mL)。大鼠经SGB阻滞2小时后处死,分离海马。HE 染色法观察海马组织的病理变化,TUNEL 染色法观察海马神经元的凋亡,免疫组化法检测海马组织中 B 细胞淋巴瘤-2 相关 X(Bax)的阳性表达,qRT-PCR 法检测海马组织中 Bax mRNA 的表达。结果显示与对照组相比,模型组海马组织损伤严重,海马神经元凋亡率、Bax蛋白阳性率、Bax mRNA表达量、Bax蛋白表达量均明显升高,Bcl-2蛋白表达量明显降低(P < 0.05).与模型组相比,SGB组海马组织损伤明显减轻,海马神经元凋亡率、Bax蛋白阳性率、Bax mRNA表达量、Bax蛋白表达量均明显降低,Bcl-2蛋白表达量明显升高(P < 0.05).与模型组相比,SGB组海马组织损伤明显减轻,海马神经元凋亡率、Bax蛋白阳性率、Bax mRNA表达量、Bax蛋白表达量均明显降低,Bcl-2蛋白表达量明显升高(P < 0.05).结论SGB 可通过抑制 Bax 基因的表达,减少大鼠神经元的凋亡,改善脑损伤。
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来源期刊
Wireless Personal Communications
Wireless Personal Communications 工程技术-电信学
CiteScore
5.80
自引率
9.10%
发文量
663
审稿时长
6.8 months
期刊介绍: The Journal on Mobile Communication and Computing ... Publishes tutorial, survey, and original research papers addressing mobile communications and computing; Investigates theoretical, engineering, and experimental aspects of radio communications, voice, data, images, and multimedia; Explores propagation, system models, speech and image coding, multiple access techniques, protocols, performance evaluation, radio local area networks, and networking and architectures, etc.; 98% of authors who answered a survey reported that they would definitely publish or probably publish in the journal again. Wireless Personal Communications is an archival, peer reviewed, scientific and technical journal addressing mobile communications and computing. It investigates theoretical, engineering, and experimental aspects of radio communications, voice, data, images, and multimedia. A partial list of topics included in the journal is: propagation, system models, speech and image coding, multiple access techniques, protocols performance evaluation, radio local area networks, and networking and architectures. In addition to the above mentioned areas, the journal also accepts papers that deal with interdisciplinary aspects of wireless communications along with: big data and analytics, business and economy, society, and the environment. The journal features five principal types of papers: full technical papers, short papers, technical aspects of policy and standardization, letters offering new research thoughts and experimental ideas, and invited papers on important and emerging topics authored by renowned experts.
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