{"title":"Mechanism of Stellate Ganglion Block Anesthesia Inhibiting Neuronal Apoptosis in Rats by Down-regulating Bax gene","authors":"Qiao Xu, Lei Li, Ke Yan, Siqi Ye","doi":"10.1007/s11277-024-11468-7","DOIUrl":null,"url":null,"abstract":"<p>To explore the feasibility of stellate ganglion block (SGB) for the treatment of brain injury and the inhibitory effect of neuronal apoptosis. SD rats were randomly divided into control group, model group (LPS 0.6 mg/kg), and SGB group (LPS 0.6 mg/kg + lidocaine 0.2 mL). The rats were sacrificed after SGB block for 2 h to separate the hippocampus. The pathological changes of hippocampal tissue were observed by HE staining, the apoptosis of hippocampal neurons was observed by TUNEL staining, the positive expression of B-cell lymphoma-2-Associated X (Bax) in hippocampal tissue was detected by immunohistochemistry, and the expression of Bax mRNA in hippocampal tissue was detected by qRT-PCR method. Results: Compared with the control group, the hippocampal tissue of the model group was severely damaged, and the apoptosis rate of hippocampal neurons, the positive rate of Bax protein, the expression of Bax mRNA, and the protein expressions of Baxwere significantly increased, the protein expression of Bcl-2 was significantly decreased (<i>P</i> < 0.05).Compared with the model group, the hippocampal tissue damage of the SGB group was significantly reduced, and the hippocampal neuron apoptosis rate, Bax protein positive rate, Bax mRNA expression and Bax protein expressions were significantly decreased, and the expression of Bcl-2 protein was significantly increased (<i>P</i> < 0.05). Conclusion: SGB can reduce the apoptosis of neurons in rats and improve brain injury by inhibiting the expression of Bax gene.</p>","PeriodicalId":23827,"journal":{"name":"Wireless Personal Communications","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Wireless Personal Communications","FirstCategoryId":"94","ListUrlMain":"https://doi.org/10.1007/s11277-024-11468-7","RegionNum":4,"RegionCategory":"计算机科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"TELECOMMUNICATIONS","Score":null,"Total":0}
引用次数: 0
Abstract
To explore the feasibility of stellate ganglion block (SGB) for the treatment of brain injury and the inhibitory effect of neuronal apoptosis. SD rats were randomly divided into control group, model group (LPS 0.6 mg/kg), and SGB group (LPS 0.6 mg/kg + lidocaine 0.2 mL). The rats were sacrificed after SGB block for 2 h to separate the hippocampus. The pathological changes of hippocampal tissue were observed by HE staining, the apoptosis of hippocampal neurons was observed by TUNEL staining, the positive expression of B-cell lymphoma-2-Associated X (Bax) in hippocampal tissue was detected by immunohistochemistry, and the expression of Bax mRNA in hippocampal tissue was detected by qRT-PCR method. Results: Compared with the control group, the hippocampal tissue of the model group was severely damaged, and the apoptosis rate of hippocampal neurons, the positive rate of Bax protein, the expression of Bax mRNA, and the protein expressions of Baxwere significantly increased, the protein expression of Bcl-2 was significantly decreased (P < 0.05).Compared with the model group, the hippocampal tissue damage of the SGB group was significantly reduced, and the hippocampal neuron apoptosis rate, Bax protein positive rate, Bax mRNA expression and Bax protein expressions were significantly decreased, and the expression of Bcl-2 protein was significantly increased (P < 0.05). Conclusion: SGB can reduce the apoptosis of neurons in rats and improve brain injury by inhibiting the expression of Bax gene.
期刊介绍:
The Journal on Mobile Communication and Computing ...
Publishes tutorial, survey, and original research papers addressing mobile communications and computing;
Investigates theoretical, engineering, and experimental aspects of radio communications, voice, data, images, and multimedia;
Explores propagation, system models, speech and image coding, multiple access techniques, protocols, performance evaluation, radio local area networks, and networking and architectures, etc.;
98% of authors who answered a survey reported that they would definitely publish or probably publish in the journal again.
Wireless Personal Communications is an archival, peer reviewed, scientific and technical journal addressing mobile communications and computing. It investigates theoretical, engineering, and experimental aspects of radio communications, voice, data, images, and multimedia. A partial list of topics included in the journal is: propagation, system models, speech and image coding, multiple access techniques, protocols performance evaluation, radio local area networks, and networking and architectures.
In addition to the above mentioned areas, the journal also accepts papers that deal with interdisciplinary aspects of wireless communications along with: big data and analytics, business and economy, society, and the environment.
The journal features five principal types of papers: full technical papers, short papers, technical aspects of policy and standardization, letters offering new research thoughts and experimental ideas, and invited papers on important and emerging topics authored by renowned experts.