Formation of leukotriene B4, 20-hydroxy leukotriene B4 and other arachidonic acid metabolites by macrophages during peritonitis in patients with continuous ambulatory peritoneal dialysis

F.J. Zijlstra , A.M.W.C. van den Broek , J.E. Vincent , P.P.H.M. Diderich , A.M.C. op de Hoek-Fez , M. Claeys
{"title":"Formation of leukotriene B4, 20-hydroxy leukotriene B4 and other arachidonic acid metabolites by macrophages during peritonitis in patients with continuous ambulatory peritoneal dialysis","authors":"F.J. Zijlstra ,&nbsp;A.M.W.C. van den Broek ,&nbsp;J.E. Vincent ,&nbsp;P.P.H.M. Diderich ,&nbsp;A.M.C. op de Hoek-Fez ,&nbsp;M. Claeys","doi":"10.1016/0262-1746(87)90067-9","DOIUrl":null,"url":null,"abstract":"<div><p>Macrophages, isolated from dialysis fluid of three patients with continuous ambulatory peritoneal dialysis (CAPD) at different times during peritonitis were labelled with <sup>14</sup>C-arachidonic acid and stimulated with the calcium ionophore A23187. The main metabolites formed by 5-lipoxygenase activity were leukotriene B<sub>4</sub> (LTB<sub>4</sub>) and 5-hydroxy-6,9,11,14-eicosatetraenoic acid (5-HETE). Smaller amounts of cyclooxygenase metabolites were present and also a major compound with an elution time between 6-keto-prostaglandin F<sub>1α</sub> (6-keto-PGF<sub>1α</sub> and thromboxane B<sub>2</sub> (TxB<sub>2</sub>). This substance was isolated, analysed by GC-MS and identified as 20-hydroxy-leukotriene B<sub>4</sub> (20-OH-LTB<sub>4</sub>). This indicates that human peritoneal macrophages obtained from CAPD not only produce leukotrienes and prostaglandins, but also the ω-hydroxylase product of LTB<sub>4</sub>, which has been demonstrated to be present in polymorphonuclear leucocytes. The activity of this enzyme was not correlated with the severity of the peritonitis.</p></div>","PeriodicalId":20720,"journal":{"name":"Prostaglandins, leukotrienes, and medicine","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1987-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0262-1746(87)90067-9","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Prostaglandins, leukotrienes, and medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0262174687900679","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 6

Abstract

Macrophages, isolated from dialysis fluid of three patients with continuous ambulatory peritoneal dialysis (CAPD) at different times during peritonitis were labelled with 14C-arachidonic acid and stimulated with the calcium ionophore A23187. The main metabolites formed by 5-lipoxygenase activity were leukotriene B4 (LTB4) and 5-hydroxy-6,9,11,14-eicosatetraenoic acid (5-HETE). Smaller amounts of cyclooxygenase metabolites were present and also a major compound with an elution time between 6-keto-prostaglandin F (6-keto-PGF and thromboxane B2 (TxB2). This substance was isolated, analysed by GC-MS and identified as 20-hydroxy-leukotriene B4 (20-OH-LTB4). This indicates that human peritoneal macrophages obtained from CAPD not only produce leukotrienes and prostaglandins, but also the ω-hydroxylase product of LTB4, which has been demonstrated to be present in polymorphonuclear leucocytes. The activity of this enzyme was not correlated with the severity of the peritonitis.

持续动态腹膜透析患者腹膜炎期间巨噬细胞形成白三烯B4、20-羟基白三烯B4及其他花生四烯酸代谢物的研究
用14c -花生四烯酸标记3例连续动态腹膜透析(CAPD)患者腹膜炎期间不同时间透析液中分离的巨噬细胞,并用钙离子载体A23187刺激巨噬细胞。5-脂氧合酶活性形成的主要代谢产物是白三烯B4 (LTB4)和5-羟基-6,9,11,14-二十碳四烯酸(5-HETE)。环加氧酶代谢物较少,洗脱时间介于6-酮-前列腺素F1α(6-酮- pgf1 α)和血栓素B2 (TxB2)之间的主要化合物。该物质经GC-MS分离鉴定为20-羟基白三烯B4 (20-OH-LTB4)。这表明从CAPD获得的人腹腔巨噬细胞不仅产生白三烯和前列腺素,而且还产生LTB4的ω-羟化酶产物,该产物已被证明存在于多形核白细胞中。该酶的活性与腹膜炎的严重程度无关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信