Immunohistochemical Expression and Histopathological Role of CD47 in Colorectal Cancer in Iraqi Patients

Fatima O Abd alkareem, Ban J. Mohamad
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Abstract

Background: Bowel cancer is the most prevalent digestive system cancer and is the 4th largest cause of cancer-related death worldwide. In Iraq, colon and rectal cancer (CRC) is the 6th most common malignancy in males and the 5th in females. This cancer is sluggish in growth, which gives a window of opportunity to screen for both precursor lesions and early cancer. The Cluster of Differentiation 47 (CD47) protein is a type of transmembrane glycoproteins found on nearly all human cells, including non-hematopoietic and hematopoietic cells. CD47 promotes CRC growth by triggering angiogenesis and apoptosis of tumor cell. Objectives: To evaluate the immunohistochemical expression of (CD47) in various colorectal samples from Iraqi patients with CRC by immunohistochemistry (IHC) assay. Specimens and methods: A total of 45 paraffin-embedded CRC tissue specimens and clinical data were obtained from the Medical City Teaching Hospital in Baghdad and a number of private laboratories in Baghdad, Iraq. In addition, 30 control colon and rectum tissues with no significant pathology were collected from the Forensic Medicine Department for comparison purposes after taking the official approvals. Results: The results revealed a high expression of CD47 in CRC cases, but with no significant correlation with clinicopathological features. Also, the result of figures in this study revealed negative membranous expression of CD47 (score 0), strong membranous expression of CD47 (score 3), moderately membranous expression of CD47 (score 2), and weak membranous expression of CD47 (score 1) Conclusion: Patients with CRC had high CD47 expression, allowing tumor cells to modulate CD47SIRP inhibitory signaling and prevent immune cell attack. 
伊拉克患者结直肠癌中 CD47 的免疫组化表达和组织病理学作用
背景:肠癌是最常见的消化系统癌症,也是全球第四大癌症致死原因。在伊拉克,结肠癌和直肠癌(CRC)在男性最常见的恶性肿瘤中排名第六,在女性中排名第五。这种癌症生长缓慢,为筛查前驱病变和早期癌症提供了机会。分化簇 47(CD47)蛋白是一种跨膜糖蛋白,几乎存在于所有人类细胞上,包括非造血细胞和造血细胞。CD47 通过触发血管生成和肿瘤细胞凋亡促进 CRC 生长:目的:通过免疫组化(IHC)方法评估(CD47)在伊拉克 CRC 患者各种结直肠样本中的免疫组化表达:标本和方法:总共 45 份石蜡包埋的 CRC 组织标本和临床数据来自伊拉克巴格达医学城教学医院和巴格达的一些私人实验室。此外,经官方批准后,还从法医部门收集了 30 份无明显病理变化的对照结肠和直肠组织进行对比:结果:研究结果显示,CD47 在 CRC 病例中高表达,但与临床病理特征无明显相关性。此外,本研究的数字结果显示 CD47 膜阴性表达(0 分)、CD47 膜强表达(3 分)、CD47 膜中度表达(2 分)和 CD47 膜弱表达(1 分):结论:CRC 患者的 CD47 高表达可使肿瘤细胞调节 CD47SIRP 抑制信号,阻止免疫细胞的攻击。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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