Seasonal Survey of Bacterial Species and their Bioremediation Potentials in Leachates from a Nigerian Municipal Solid Waste Dumpsite

Abstract

Myriads of microorganisms present in dumpsite leachates produce varieties of extracellular enzymes that can degrade toxic compounds to innocuous products. The present study involved a seasonal survey of bacterial species and their bioremediation potentials in leachate from the Ikueniro open solid waste dumpsite situated in Uhunmwode Local Government Area, Edo State, Nigeria. Bacterial isolation and enumeration of the leachate samples was carried out using pour plate technique. Phenotypic techniques and 16S rRNA gene analysis identified the bacterial isolates. The bacterial mastermix for bioremediation of the leachate consisted of a consortium of bacterial strains isolated from leachate discharging from the Ikhueniro dumpsite. The bioremediation efficiency of the leachate was deduced by measurement of heavy metals and other chemical analytes using standard methods prescribed by the American Public Health Association. The bacterial species isolated from Ikueniro dumpsite such as Bacillus licheniformis strain EGBON & OKORIE 103 and Klebsiella aerogenes strain EGBON & OKORIE 108 were deposited in the United States NCBI GenBank. A consortium of these bacterial strains served as inocula for the bioremediation setup. A huge removal of ammonia from the leachate was observed during the 28-day bioremediation experiment, amounting to a mean bioremediation efficiency of 80.65%. The removal of the heavy metals from the raw leachate by the bacterial mastermix in increasing order was as follows: nickel

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尼日利亚城市固体废物倾倒场渗滤液中细菌种类及其生物修复潜力的季节性调查

垃圾场沥滤液中存在大量微生物，它们能产生各种胞外酶，将有毒化合物降解为无害产物。本研究对尼日利亚埃多州乌洪姆沃德地方政府辖区 Ikueniro 露天固体废弃物倾倒场沥滤液中的细菌种类及其生物修复潜力进行了季节性调查。采用倾板技术对沥滤液样本进行了细菌分离和计数。表型技术和 16S rRNA 基因分析确定了细菌分离物。用于沥滤液生物修复的细菌主混合物由从 Ikhueniro 垃圾场排放的沥滤液中分离出来的细菌菌株组成。沥滤液的生物修复效率是通过使用美国公共卫生协会规定的标准方法测量重金属和其他化学分析物推断出来的。从 Ikueniro 垃圾场分离出的地衣芽孢杆菌菌株 EGBON & OKORIE 103 和产气克雷伯氏菌菌株 EGBON & OKORIE 108 等细菌物种已存入美国 NCBI GenBank。这些细菌菌株组成的菌群被用作生物修复装置的接种菌。在为期 28 天的生物修复实验中，渗滤液中的氨被大量去除，平均生物修复效率达到 80.65%。细菌母液对原始沥滤液中重金属的去除率依次为：镍<铜<锌<铅<铁<镉<砷<汞。利用本地细菌对沥滤液进行生物修复，有望成为管理露天固体废物倾倒场沥滤液中有毒物质的有效工具。

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