First Report of Human Parainfluenza Viruses among Patients with Respiratory Symptoms in Khartoum State, Sudan

International Journal of Science and Healthcare Research Pub Date : 2024-07-01 DOI:10.52403/ijshr.20240255

Awadalkareem Y. Awadalkareem, Abdel Rahim M El Hussein, Naglaa M Ahmed

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Abstract

Background: This study was carried out to detect human Parainfluenza virus 'HPIV' RNA in patients with respiratory infections in Khartoum state in order to determine the circulating types of Parainfluenza virus 'in Khartoum state, Sudan. In addition, the study aimed to locally establish the diagnosis of parainfluenza virus using of RT-PCR. Methods: The study was conducted in Khartoum state Hospitals during the period from January 2019 to February 2021. Nasopharyngeal swabs samples were collected from 149 patients with respiratory symptoms. Samples were processed for RNA extraction and virus isolation. Extracted RNA was subjected to RT-PCR for the detection of Parainfluenza virus RNA and to further determine Parainfluenza virus serotypes. Positive samples were inoculated in chicken embryonated egg to isolate the virus. Results: Out of 149 samples 13 samples (8.7%) were positive and 136 samples (91.3%) were negative for parainfluenza virus RNA using RT-PCR. Parainfluenza types 1 and 2 were not detected while parainfluenza-3 was detected in 8 sample (5.4%) and parainfluenza-4 was positive in 5 samples (3.4%). Among PIV RNA positive samples (n=13); no virus was isolated using embryonated eggs inoculation. Conclusion: Existence and incidence of human parainfluenza virus and its types in Sudan was documented through detection of Human PIV(HPIV) RNA in respiratory samples. Moreover, the HPIV detection using RT-PCR was established. Keywords: Human Parainfluenza, Serotypes, RT-PCR, Emberyonated egg.

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苏丹喀土穆州首次报告有呼吸道症状的患者感染了人类副流感病毒

研究背景本研究旨在检测喀土穆州呼吸道感染患者体内的人副流感病毒（HPIV）RNA，以确定苏丹喀土穆州副流感病毒的流行类型。此外，该研究还旨在利用 RT-PCR 技术在当地对副流感病毒进行诊断：研究于 2019 年 1 月至 2021 年 2 月期间在喀土穆州医院进行。从 149 名有呼吸道症状的患者身上采集了鼻咽拭子样本。样本经处理后进行 RNA 提取和病毒分离。提取的 RNA 经 RT-PCR 检测副流感病毒 RNA，并进一步确定副流感病毒血清型。将阳性样本接种到鸡胚蛋中以分离病毒：在 149 个样本中，13 个样本（8.7%）的副流感病毒 RNA 呈阳性，136 个样本（91.3%）呈阴性。未检测到 1 型和 2 型副流感病毒，8 个样本（5.4%）检测到 3 型副流感病毒，5 个样本（3.4%）检测到 4 型副流感病毒。在 PIV RNA 阳性的样本中（n=13），使用胚胎卵接种没有分离到病毒：结论：通过检测呼吸道样本中的人类副流感病毒（HPIV）RNA，证实了苏丹存在人类副流感病毒及其类型，且发病率较高。此外，还利用 RT-PCR 技术确定了 HPIV 的检测方法：人副流感血清型 RT-PCR 胚胎蛋

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International Journal of Science and Healthcare Research

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