Optimization of Enzymatic Assisted Extraction of Bioactive Compounds from Olea europaea Leaves

Biomass Pub Date : 2024-07-01 DOI:10.3390/biomass4030035

Alexios Vardakas, Achilleas Kechagias, Nikolay Penov, A. Giannakas

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Abstract

Nowadays, the circular economy trend drives researchers in the recovery of various bioactive compounds from agri-food by-products. Enzyme-assisted extraction (EAE) has been shown to be an innovative green technology for the effective extraction of various phytochemicals from agri-food section by-products; therefore, this study aimed to evaluate the application of EAE as green technology to obtain extracts from olive leaves (Olea europaea) for potential industrial production. The used enzymes were Celluclast, Pectinex XXL and Viscozyme L. EAE was conducted under various enzyme dose combinations and an incubation time of 120 min. Obtained extracts were characterized in terms of total polyphenols (TP) and total antioxidant activity (AA). Firstly, the enzyme synergistic effect in the enzymatic extraction of polyphenols was evaluated. TP optimal extraction conditions (468.19 mg GAE (gallic acid equivalent)/L of extract) were achieved after EAE using Pectinex and Viscozyme enzymes (50–50 v/v) and for AA (69.85 AA%). According to the above results, a second experiment investigated the effect of incubation time (min.) and enzyme dose (mL) on the optimal extraction conditions of olive leaves. The final results after optimization were 75% higher than the control sample for the TP content (605.55 mg GAE/L) and 8% higher for the AA (70.14 AA%). These results indicated that EAE is an excellent choice for the green extraction of polyphenols from the olive leaves.

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优化从油橄榄叶中提取生物活性化合物的酶辅助提取方法

如今，循环经济的趋势推动着研究人员从农业食品副产品中回收各种生物活性化合物。酶辅助萃取（EAE）已被证明是一种创新的绿色技术，可从农业食品部分的副产品中有效提取各种植物化学物质；因此，本研究旨在评估酶辅助萃取作为绿色技术在从橄榄叶（Olea europaea）中获取萃取物的潜在工业生产中的应用。使用的酶是 Celluclast、Pectinex XXL 和 Viscozyme L。EAE 在不同的酶剂量组合和 120 分钟的培养时间下进行。提取物的特征是总多酚（TP）和总抗氧化活性（AA）。首先，评估了酶法提取多酚的酶协同效应。使用 Pectinex 和 Viscozyme 酶（50-50 v/v）进行 EAE 提取后，TP 达到最佳提取条件（468.19 mg GAE（没食子酸当量）/L 提取液），AA 达到最佳提取条件（69.85 AA%）。根据上述结果，第二次实验研究了孵育时间（分钟）和酶剂量（毫升）对橄榄叶最佳提取条件的影响。优化后的最终结果是 TP 含量（605.55 mg GAE/L）比对照样品高 75%，AA 含量（70.14 AA%）比对照样品高 8%。这些结果表明，EAE 是从橄榄叶中绿色提取多酚的最佳选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biomass

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