Preparation, drug loading and antibacterial properties of zinc-iron-based nanomaterials

Abstract

Objective: To explore the preparation of a nano-scale zinc-iron-based material, and discuss the antibacterial and drug-loading properties of the nano-material. Methods: (1) Preparation and analysis of nanomaterials: On the basis of the preparation of Prussian substance, magnetic zinc-iron nanomaterials were prepared using this substance as a precursor. The shape and topography of the zinc-iron-based materials are analyzed by transmission electron microscope equipment and scanning electron microscope equipment, and their composition is analyzed by X-ray method. (2) Analysis of drug-loading properties of nanomaterials: Tetracycline and capsaicin were used as drug-loading analysis models to study the adsorption and release effects of materials on the two carriers. (3) Toxicity test analysis: The thiazolium blue colorimetric method was used to analyze the influence relationship between the drug-loaded particles loaded with capsaicin and HepG-2 cells, and the influence results were analyzed. (4) Antibacterial performance analysis: study the drug-loaded particles loaded with tetracycline and observe the inhibitory effect of the carrier on the growth of the strain. Results: The Zn3[Fe(CN)6]2 xH2O sample material was prepared from K3Fe(CN)6 and ZnCl2, and the self-made sample was detected by the X-ray analyzer. The sample conformed to the high-purity Zn3[Fe(CN)6]2 ·xH2O standard. And the self-made Zn3[Fe(CN)6]2 ·xH2O was analyzed for drug loading, and the release of capsaicin can reach 86.3% under a certain phosphate buffer solution. At the same time, Zn3[Fe(CN)6]2 · xH2O supports tetracycline, and the maximum release rate can reach 90% in phosphate buffer. The self-made Zn3[Fe(CN)6]2 ·xH2O samples and ZnFe2O4/ZnO were tested for antibacterial properties, and the Zn3[Fe(CN)6]2 · xH2O samples were loaded with after capsaicin has a significant inhibitory effect on cancer cells, the inhibitory effect is more obvious than other groups. At the same time, tetracycline was loaded on ZnFe2O4/ZnO for antibacterial experiments, and the inhibitory effect of the drug loading on the bacteria was more obvious. Conclusion: Zinc-iron-based nanomaterials can be successfully prepared by K3Fe(CN)6 and ZnCl2 raw materials. During the preparation, it is necessary to control the time and the concentration of preparation agents reasonably to ensure the quality of the samples. The two samples prepared have good effect adsorption in terms of drug loading, and the application of specific drug models at the same time has better performance in antibacterial effect. The research content provides corresponding technical references for the application of material technology in the medical field.