The effectiveness of semi-wild Sumatran mango (Mangifera spp.) leaves as a phytotherapy agent for breast cancer

IF 0.7 Q4 PHARMACOLOGY & PHARMACY
Fitmawati, Nur Anisa, R. Roza, E. Juliantari
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引用次数: 0

Abstract

Breast cancer ranks first in the world, standing at a mortality rate of 24.5% per year and is the leading cause of cancer death in Indonesia. The current management of breast cancer therapy is considered less effective because of its careful use due to side effects that are detrimental to the patient. The semi-wild species from Sumatra are neglected and underutilized species but have the potential as a therapeutic agent. Previous research has revealed that this species of mango is high in antioxidant compounds. This study was carried out to discover the anticancer activities of the semi-wild mango species via inhibitory activities and morphological changes in Michigan Cancer Foundation-7 (MCF-7) cells. The IC50 value of Mangifera sumatrana, Mangifera foetida, and Mangifera laurina leaves in n-hexane, ethyl-acetate, and methanol extracts was determined using the reagent 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Data were analyzed by two-way analysis of variance using IBM SPSS Statistics 21. M. laurina n-hexane extracts exhibited anticancer activity (IC50 13.25 ppm). Nonpolar solutions were chosen as the most effective extraction solvent in anticancer tests because lipids in nonpolar solvents can hit the lipid bilayer. A hexane fraction was created by separating the majority of nonpolar fatty acid esters, and this fraction had a considerable impact on cytotoxic and apoptotic effects on MCF-7 cells. Therefore, all treatments can transform MCF-7 cells’ morphology into blackened dead cells that are degraded into small parts, such as apoptotic bodies in cells undergoing apoptotic processes. MTT assays against MCF-7 on three species of semi-wild Sumatran mango in different extraction solvents showed that n-hexane extracts of M. laurina had stronger anticancer activity than other samples. This study provides new information to support the development of standardized herbal medicines and phytopharmaca in the future.
半野生苏门答腊芒果(Mangifera spp.)
乳腺癌的死亡率居世界首位,每年高达 24.5%,是印度尼西亚癌症死亡的主要原因。目前的乳腺癌治疗方法被认为效果不佳,因为副作用对患者不利,所以必须谨慎使用。苏门答腊岛的半野生物种是被忽视和利用不足的物种,但具有作为治疗药物的潜力。以前的研究表明,这种芒果含有大量抗氧化化合物。 本研究通过密歇根癌症基金会-7(MCF-7)细胞的抑制活性和形态变化来发现半野生芒果物种的抗癌活性。 使用试剂 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑(MTT)测定了苏门答腊芒果、佛手芒果和月芒叶在正己烷、乙酸乙酯和甲醇提取物中的 IC50 值。数据采用 IBM SPSS Statistics 21 进行双向方差分析。 月桂树正己烷提取物具有抗癌活性(IC50 13.25 ppm)。在抗癌试验中,非极性溶液被选为最有效的提取溶剂,因为非极性溶剂中的脂质可以撞击脂质双分子层。通过分离大部分非极性脂肪酸酯,产生了正己烷馏分,该馏分对 MCF-7 细胞的细胞毒性和凋亡效应有相当大的影响。因此,所有处理方法都能将 MCF-7 细胞的形态转变为变黑的死细胞,并降解成小部分,如细胞凋亡过程中的凋亡体。对三种半野生苏门答腊芒果在不同提取溶剂中的 MCF-7 进行的 MTT 分析表明,月桂芒果的正己烷提取物比其他样品具有更强的抗癌活性。这项研究为今后开发标准化草药和植物药提供了新的信息。
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来源期刊
Egyptian Pharmaceutical Journal
Egyptian Pharmaceutical Journal PHARMACOLOGY & PHARMACY-
CiteScore
1.10
自引率
0.00%
发文量
37
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