Natural pyrethrins induced developmental toxicity of zebrafish swim bladder in vivo and genotoxicity of lung cells in vitro

IF 2.6 3区医学 Q3 TOXICOLOGY

Toxicology in Vitro Pub Date : 2024-07-16 DOI:10.1016/j.tiv.2024.105896

Ruirui Li , Cheng Zhang , Wenping Xu , Liming Tao , Jiagao Cheng , Zhong Li , Yang Zhang

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Abstract

Natural pyrethrins (NPs) are insecticidal compounds isolated and extracted from pyrethrum flowers and are primarily use to control sanitary pests. The lungs become the main target after exposure, and its use may pose potential hazards to respiratory health. Therefore, in this paper, the toxic effects of NPs on human lung cells A549 were investigated and the risk of respiratory toxicity of NPs was studied using zebrafish swim bladder as a model. The results showed that NPs induced cytotoxicity, caused oxidative DNA damage and triggered mitochondria-mediated apoptosis. In addition, exposure to NPs decreased zebrafish embryo survival, hatchability, and heartbeat, and may inhibit normal swim bladder development by disrupting Wnt and Hedgehog signaling pathways. In conclusion, our results suggest that NPs can induce cytotoxicity in A549 in vitro and developmental toxicity in zebrafish in vivo. This study provides a conceptual basis for understanding the mechanisms of toxicity of NPs and assessing respiratory health risks in humans.

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天然除虫菊酯诱导斑马鱼鳔的体内发育毒性和肺细胞的体外遗传毒性

天然除虫菊酯（NPs）是从除虫菊花中分离和提取的杀虫化合物，主要用于控制卫生害虫。接触NPs后，肺部成为主要靶标，使用NPs可能会对呼吸系统健康造成潜在危害。因此，本文研究了 NPs 对人肺部细胞 A549 的毒性作用，并以斑马鱼鳔为模型研究了 NPs 的呼吸道毒性风险。结果表明，NPs 会诱导细胞毒性，造成 DNA 氧化损伤，并引发线粒体介导的细胞凋亡。此外，暴露于 NPs 会降低斑马鱼胚胎的存活率、孵化率和心跳，并可能通过破坏 Wnt 和刺猬信号通路抑制鳔的正常发育。总之，我们的研究结果表明，NPs 在体外可诱导 A549 的细胞毒性，在体内可诱导斑马鱼的发育毒性。这项研究为了解 NPs 的毒性机制和评估人类呼吸系统健康风险提供了概念基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicology in Vitro 医学-毒理学

CiteScore

6.50

自引率

3.10%

发文量

181

审稿时长

65 days

期刊介绍： Toxicology in Vitro publishes original research papers and reviews on the application and use of in vitro systems for assessing or predicting the toxic effects of chemicals and elucidating their mechanisms of action. These in vitro techniques include utilizing cell or tissue cultures, isolated cells, tissue slices, subcellular fractions, transgenic cell cultures, and cells from transgenic organisms, as well as in silico modelling. The Journal will focus on investigations that involve the development and validation of new in vitro methods, e.g. for prediction of toxic effects based on traditional and in silico modelling; on the use of methods in high-throughput toxicology and pharmacology; elucidation of mechanisms of toxic action; the application of genomics, transcriptomics and proteomics in toxicology, as well as on comparative studies that characterise the relationship between in vitro and in vivo findings. The Journal strongly encourages the submission of manuscripts that focus on the development of in vitro methods, their practical applications and regulatory use (e.g. in the areas of food components cosmetics, pharmaceuticals, pesticides, and industrial chemicals). Toxicology in Vitro discourages papers that record reporting on toxicological effects from materials, such as plant extracts or herbal medicines, that have not been chemically characterized.