First Genotyping of Mycobacterium avium Subspecies Paratuberculosis from Large Ruminant Population at Satna District in Madhya Pradesh in India to Bison Type

Indian Journal of Animal Research Pub Date : 2024-07-03 DOI:10.18805/ijar.b-5318

S. Audarya, K. Chaubey, S. Gupta, B. Bharti, N. Pathak, D. Chhabra, S. Matoli, A.K. Mishra, S.V. Singh

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Abstract

Background: Paratuberculosis or Johne’s disease in large ruminants is caused due to Mycobacterium avium subspecies paratuberculosis. The bacterial disease in large ruminants is characterized by chronic diarrhea, weakness and emaciation. There are no reports on Paratuberculosis in this area’s large ruminant population and its genotyping. Methods: A total of 76 samples (26 fecal and 50 milk) of large ruminants from Satna district in Madhya Pradesh were investigated (microscopical; for the presence of acid-fast organisms and molecular methods; for gene-specific amplification) for the presence of Mycobacterium avium subspecies paratuberculosis infection. Result: Microscopical examinations of fecal samples revealed 11.54% positivity and milk samples 14% positivity of Mycobacterium avium subspecies paratuberculosis infection. A single fecal and 2 milk samples were found positive in the IS900 polymerase chain reaction test (413 bp product). Specific amplification of 608 bp IS1311 product followed by restriction endonuclease analysis proved the genotype of Mycobacterium avium subspecies paratuberculosis as Bison type.

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首次从印度中央邦萨特纳县的大型反刍动物种群中鉴定出副结核分枝杆菌的基因型，并将其归类为野牛型分枝杆菌

背景：大型反刍动物的副结核病或约翰氏病是由副结核分枝杆菌引起的。这种细菌性疾病在大型反刍动物中的特征是慢性腹泻、虚弱和消瘦。目前还没有关于该地区大型反刍动物副结核病及其基因分型的报告。研究方法对中央邦萨特纳地区的 76 份大型反刍动物样本（26 份粪便样本和 50 份牛奶样本）进行了调查（显微镜检查；检测是否存在耐酸菌；分子方法；检测基因特异性扩增），以确定是否存在副结核分枝杆菌感染。结果：粪便样本显微镜检查显示 11.54%的阳性率，牛奶样本显示 14%的阳性率。在 IS900 聚合酶链反应测试中，发现一个粪便样本和两个牛奶样本呈阳性（413 bp 产物）。对 608 bp 的 IS1311 产物进行特异性扩增，然后进行限制性内切酶分析，证明副结核分枝杆菌的基因型为野牛型。

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Indian Journal of Animal Research

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