Topographic analysis of fiber posts after surface disinfection with different methods

Victoria Melcchiades Baltezan, Gustavo Golgo Kunert, Tiago Melo Tiago André Fontoura de Melo
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Abstract

Aims: This study aims to comprehensively examine the surface morphology of fiber posts after undergoing various disinfection methods, utilizing scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). Materials and Methods: Twenty-one fiber posts were randomly allocated into seven experimental groups, each consisting of three samples. The disinfection methods employed were as follows: GC - no disinfection treatment; GAL - immersion in 70% alcohol, following the manufacturer's recommended protocol; GHP - soaking in 2.5% sodium hypochlorite for a duration of 10 minutes; GCL - soaking in 2% chlorhexidine gluconate for a period of five minutes; GAC - 30-second etching with 35% phosphoric acid; GPH - soaking in 10% hydrogen peroxide for a duration of 20 minutes; and GSL - autoclave sterilization. Following the disinfection procedures, SEM was employed to scrutinize the surface topography of the posts, while EDX was utilized to identify the chemical elements present on the sample surfaces. Subsequently, a descriptive analysis was conducted on the SEM images and EDX data. Results: SEM analysis revealed that all groups exhibited regions with epoxy resin-coated fibers alongside sections with exposed glass fibers. Analysis of the EDX data indicated that there were no significant differences in the predominant chemical elements across the groups. Carbon (C) and oxygen (O) registered the highest peaks, followed by silicon (Si), zirconium (Zr), sodium (Na), aluminum (Al), and calcium (Ca). Conclusions: The disinfection methods under investigation did not induce substantial alterations in the surface morphology of the fiber posts.
用不同方法对纤维柱进行表面消毒后的地形分析
目的:本研究旨在利用扫描电子显微镜(SEM)和能量色散 X 射线光谱(EDX),全面研究纤维柱在经过各种消毒方法后的表面形态。材料和方法:21 根纤维柱被随机分为 7 个实验组,每组包括 3 个样品。采用的消毒方法如下GC--不进行消毒处理;GAL--按照制造商的建议方案,在 70% 的酒精中浸泡;GHP--在 2.5% 的次氯酸钠中浸泡 10 分钟;GCL--在 2% 的葡萄糖酸氯己定中浸泡 5 分钟;GAC--用 35% 的磷酸浸泡 30 秒;GPH--在 10% 的过氧化氢中浸泡 20 分钟;GSL--高压灭菌器消毒。消毒程序结束后,使用扫描电子显微镜(SEM)仔细检查柱子的表面形貌,并使用电子衍射X射线(EDX)鉴定样品表面存在的化学元素。随后,对 SEM 图像和 EDX 数据进行了描述性分析。结果:扫描电子显微镜分析表明,所有组别都有环氧树脂涂层纤维区域和玻璃纤维裸露部分。EDX 数据分析表明,各组的主要化学元素没有明显差异。碳(C)和氧(O)的峰值最高,其次是硅(Si)、锆(Zr)、钠(Na)、铝(Al)和钙(Ca)。结论所研究的消毒方法并未引起纤维柱表面形态的实质性改变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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