Evaluation of the Antioxidant and Antimicrobial Properties of Hydrosol Extract and Essential Oil Derived from the Aerial Parts of Salvia argentea

Q4 Medicine

Anti-Infective Agents Pub Date : 2024-07-03 DOI:10.2174/0122113525303193240606062041

I. Chikhi, Rachida Kerzabi, Safaa Nor el Houda Ziane, Fatima Benyoucef, M. A. Dib, A. Muselli

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引用次数: 0

Abstract

The increasing use of synthetic antimicrobials and antioxidants is a concern for human health. The main objective of this study was to examine the chemical composition of the essential oil extracted from Salvia argentea and its hydrosol extract while evaluating their antioxidant and antimicrobial effects in vitro. The essential oil was obtained by hydrodistillation, while the hydrosol extract was obtained by the liquid-liquid extraction method. The volatile compounds of the essential oil and hydrosol extract were analysed by gas chromatography (GC/FID) and gas chromatography coupled with mass spectrometry (GC/MS). Antioxidant activities were evaluated using two methods: free radical scavenging activity (DPPH) and iron reduction antioxidant power (FRAP), with BHT used as a positive control. The antimicrobial activity of the essential oil and hydrosol extract was evaluated using the agar disc diffusion method. According to the GC/FID and GC/MS analysis, the essential oil of S. argentea consisted mainly of germacrene D-4-ol (26.3%), epi-α-muurolol (19.2%), and epi-α-cadinol (10.2%). In contrast, the hydrosol extract consisted of oxygenated compounds, including (E)- phytol (23.5%), 14-hydroxy-δ-cadine (17.5%), caryophyllene oxide (11.5%), piperitone (13.5%), and piperitone oxide (11.5%). The essential oil and hydrosol extract showed positive antioxidant activity with both tests. It should be noted that the hydrosol extract showed the best performance, with an IC50 of 8.3 g/L with the DPPH and an IC50 of 12.3 g/L with the FRAP test compared to the BHT. The essential oil revealed antimicrobial activity with strong inhibition against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus subtilis compared to Gentamicin. The results showed that essential oil and hydrosol extract have promising antimicrobial and antioxidant properties. These findings provide encouraging prospects for their potential use in the pharmaceutical or cosmetic sectors.

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评估丹参草本植物水溶液提取物和精油的抗氧化和抗菌特性

本研究的主要目的是研究从丹参中提取的精油及其水醇提取物的化学成分，同时评估它们在体外的抗氧化和抗菌效果。精油通过水蒸馏法获得，而水醇提取物则通过液液萃取法获得。精油和水醇提取物的挥发性化合物采用气相色谱法（GC/FID）和气相色谱-质谱法（GC/MS）进行分析。抗氧化活性采用两种方法进行评估：自由基清除活性（DPPH）和铁还原抗氧化力（FRAP），并以 BHT 作为阳性对照。根据 GC/FID 和 GC/MS 分析，银莲花精油的主要成分为芽胞烯 D-4-醇（26.3%）、表-α-木洛尔醇（19.2%）和表-α-卡地诺醇（10.2%）。相反，水醇萃取物由含氧化合物组成，包括 (E)-phytol (23.5%)、14-hydroxy-δ-cadine (17.5%)、caryophyllene oxide (11.5%)、piperitone (13.5%) 和 piperitone oxide (11.5%)。精油和水醇提取物在这两项测试中都显示出积极的抗氧化活性。值得注意的是，与 BHT 相比，水醇提取物的表现最好，DPPH 试验的 IC50 为 8.3 克/升，FRAP 试验的 IC50 为 12.3 克/升。与庆大霉素相比，精油对大肠杆菌、金黄色葡萄球菌、绿脓杆菌和枯草杆菌具有很强的抑制作用。这些发现为它们在制药或化妆品领域的潜在用途提供了令人鼓舞的前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anti-Infective Agents Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

1.50

自引率

0.00%

发文量

期刊介绍： Anti-Infective Agents publishes original research articles, full-length/mini reviews, drug clinical trial studies and guest edited issues on all the latest and outstanding developments on the medicinal chemistry, biology, pharmacology and use of anti-infective and anti-parasitic agents. The scope of the journal covers all pre-clinical and clinical research on antimicrobials, antibacterials, antiviral, antifungal, and antiparasitic agents. Anti-Infective Agents is an essential journal for all infectious disease researchers in industry, academia and the health services.