ASSESSMENT OF THE ENZYMES EFFECTIVENESS USED IN STARCH SYRUP PRODUCTION

Dar'ya Koval', Anna Maslennikova, Ekaterina Saharova, Elena Vlasova
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Abstract

The purpose of research is to study the influence of amylolytic enzymes on organoleptic, physicoche-mical, microbiological and toxicological indicators of the quality of caramel syrup. Objectives: to produce caramel syrup from corn and wheat starch using enzymes: α-amylase and glucoamylase; to determine organoleptic, physicochemical, microbiological and toxicological indicators of the quality of the resulting syrup; to identify the most effective enzyme to produce high-quality syrup. The objects of the study are the commercial enzymes α-amylase Lphera and glucoamylase Dextrozyme-1.5. It was established that according to organoleptic (taste, smell, color, transparency) and physico-chemical (mass fraction of dry and reducing substances, total ash; pH; acidity; sulfur dioxide content; caramel sample temperature; specific electrical conductivity) indicators, syrup obtained from corn starch using α-amylase, fully complies with the requirements of the state standard. Caramel syrup produced using both types of enzymes from wheat starch turned out to be cloudy. This is due to the peculiarities of the production of wheat suspension from which syrup is made. The mass fraction of dry and reducing substances in syrup obtained from both types of starch using glucoamylase is lower than normal by 5 and 14 %, respectively. The temperature value of the caramel sample of such syrup is 10 °C higher than the standard one. It has been shown that the safety indicators of syrup produced from corn and wheat starch using these enzymes meet the required stan¬dards. It has been established that the α-amylase enzyme is suitable for the production of high-quality ca¬ramel syrup that meets all the requirements of the state standard.
评估淀粉糖浆生产中使用的酶的有效性
研究目的:研究淀粉分解酶对焦糖糖浆质量的感官、理化、微生物和毒理学指标的影响。目标:使用酶:α-淀粉酶和葡萄糖淀粉酶从玉米和小麦淀粉中生产焦糖糖浆;确定所得糖浆质量的感官、理化、微生物和毒理学指标;确定生产优质糖浆的最有效酶。研究对象是商品酶 α-淀粉酶 Lphera 和葡萄糖淀粉酶 Dextrozyme-1.5。根据感官(口感、气味、颜色、透明度)和理化(干物质和还原物质的质量分数、总灰分、pH 值、酸度、二氧化硫含量、焦糖样品温度、比电导率)指标,使用 α 淀粉酶从玉米淀粉中提取的糖浆完全符合国家标准要求。使用这两种酶从小麦淀粉中生产的焦糖糖浆是浑浊的。这是由于生产糖浆的小麦悬浮液的特殊性造成的。使用葡萄糖淀粉酶从两种淀粉中获得的糖浆中,干物质和还原物质的质量分数分别比正常值低 5% 和 14%。这种糖浆的焦糖样品的温度值比标准值高 10 °C。研究表明,使用这些酶从玉米和小麦淀粉中生产糖浆的安全指标符合标准要求。已确定α-淀粉酶适用于生产符合国家标准所有要求的优质焦糖糖浆。
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