High-density genetic map construction and QTL mapping in the sorghum-sudangrass RIL population (M81×Sa)

IF 0.9 4区生物学 Q4 PLANT SCIENCES

Pakistan Journal of Botany Pub Date : 2024-07-08 DOI:10.30848/pjb2025-1(2)

Yanlong Liu, Xianlian Chen, Wen-Yao Zhao, Ling Niu, Jieqin Li, Yuan Liang, Xuehui Yan, Mengru Shen, Q. Zhan

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引用次数: 0

Abstract

Sorghum-sudangrass, a hybrid of sorghum and sudangrass has high productivity, strong adaptability, and superior quality and is widely used in animal husbandry and aquaculture. Parsing the genetic basis of major agronomic traits is very important for sorghum and sudangrass hybrid breeding. This study used a sweet sorghum M81 and sudangrass Sa hybrid to construct 174 F 11 populations. Single-nucleotide polymorphisms (SNPs) derived from genotyping using its Restriction-site Associated DNA Sequence (RAD-seq) of RILs to construct a high-density genetic map comprising 790 SNPs spanning 2101 cM, in which coverage of all ten chromosomes was greater than 80%. Inclusive Composite Interval Mapping (ICIM) was carried out to map Quantitative trait loci (QTL) for five forage traits. A total of 18 QTLs were identified for plant height (PH, 4 QTLs), fresh weight (FW, 8 QTLs), dry weight (DW, 3 QTLs), stem diameter (SD, 2 QTLs), and sugar content (SC, 1 QTL). In addition, two overlapping QTLs ( qFW9.2 / qDW9 and qFW10 / qDW10.2 ) were detected for FW and DW. Together, the RIL populations and high-density linkage maps in this study provide a theoretical basis for future sorghum-sudangrass hybrid forage breeding.

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高粱-苏丹草 RIL 群体（M81×Sa）的高密度遗传图谱构建和 QTL 图谱绘制

高粱-苏丹草是高粱和苏丹草的杂交种，具有产量高、适应性强、品质优良等特点，被广泛应用于畜牧业和水产养殖业。解析主要农艺性状的遗传基础对高粱和苏丹草杂交育种非常重要。本研究利用甜高粱 M81 和苏丹草 Sa 杂交品种构建了 174 个 F 11 群体。利用其限制位点相关 DNA 序列（RAD-seq）对 RIL 进行基因分型，获得单核苷酸多态性（SNPs），从而构建了由 790 个 SNPs 组成的高密度遗传图谱，跨度达 2101 cM，其中十条染色体的覆盖率均超过 80%。为了绘制五个饲料性状的数量性状位点（QTL）图，进行了包容性复合区间图（ICIM）。共鉴定出 18 个 QTL，包括株高（PH，4 个 QTL）、鲜重（FW，8 个 QTL）、干重（DW，3 个 QTL）、茎直径（SD，2 个 QTL）和含糖量（SC，1 个 QTL）。此外，还检测到两个重叠的 QTL（qFW9.2 / qDW9 和 qFW10 / qDW10.2），分别与 FW 和 DW 有关。本研究的 RIL 群体和高密度连接图为未来高粱-苏丹草杂交牧草育种提供了理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pakistan Journal of Botany 生物-植物科学

CiteScore

2.40

自引率

8.30%

发文量

218

审稿时长

8.4 months

期刊介绍： The Pakistan Journal of Botany is an international journal for publication of original research in plant science. Work on all plant groups, including fossil plants, is published. The journal publishes in the areas of: ecology and ecophysiology; conservation biology and biodiversity; forest biology and management; cell and molecular biology; paleobotany; reproductive biology and genetics; mycology and pathology; and structure and development.