Andrographis paniculata Ethanolic Extract Improved Doxorubicin-induced Cardiac Inflammation, Alterations in Liver Function Parameters and Anemia

Molecular and Cellular Biomedical Sciences Pub Date : 2024-07-09 DOI:10.21705/mcbs.v8i2.444

Oluebube Magnificient Eziefule, W. Arozal, S. Wanandi, M. Louisa, P. Wuyung, Syarifah Dewi, N. Nafrialdi, Yulia Ratna Dewi, Deya Adiby Nabillah

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Abstract

Background: Doxorubicin (DOX), an efficacious chemotherapy drug is compromised by cardiotoxicity, myelosuppression, and hepatotoxicity. Due to the limited success of current treatments for DOX toxicity, there is a pressing need to explore alternative medical interventions, particularly from plant sources. This study was conducted to investigate the potential protective effect of ethanolic extract of Andrographis paniculata leaves (EEAP) against DOX-induced cardiac inflammation, liver toxicity, and anemia.Materials and methods: Sprague-Dawley rats were intraperitoneally injected with DOX at a total dose of 16 mg/kgBW. EEAP was administered orally for 4 weeks at doses of 125, 250, and 500 mg/kgBW/day according to the assigned treatment groups. The mRNA expression levels of interleukin-1β (IL-1β) and nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3 (NLRP3) in the heart tissue, along with the concentrations of nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB) and calcium level were examined. Additionally, the hematological parameters (including hematocrit, hemoglobin and red blood cells (RBCs)), aspartate aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), and malondialdehyde (MDA) levels in blood were also analyzed.Results: EEAP dose-dependently decreased the mRNA expressions of IL-1β (p<0.05), tended to decrease mRNA expression of NLRP3 and the concentrations of NFκB and calcium in heart tissue compared with the DOX-only group. Additionally, EEAP dose-dependently decreased ALP values (p<0.0001) and tended to improve hematological parameters, as well as AST and MDA levels in serum.Conclusion: This extract may prevent DOX-induced cardiac inflammation, anemia, and hepatotoxicity. However, further studies are needed to confirm these findings, including the efficacy profile of the extract in cancer rats treated with DOX.Keywords: doxorubicin, Andrographis paniculata, inflammation, anemia, hepatotoxicity, herbal medicine

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穿心莲乙醇提取物可改善多柔比星诱导的心脏炎症、肝功能参数变化和贫血症

背景：多柔比星（DOX）是一种有效的化疗药物，其毒性主要表现为心脏毒性、骨髓抑制和肝毒性。由于目前治疗 DOX 毒性的方法效果有限，因此迫切需要探索替代性医疗干预措施，特别是来自植物的干预措施。本研究旨在探讨穿心莲叶乙醇提取物（EEAP）对 DOX 引起的心脏炎症、肝脏毒性和贫血的潜在保护作用：给 Sprague-Dawley 大鼠腹腔注射总剂量为 16 毫克/千克体重的 DOX。按照指定的治疗组，以 125、250 和 500 毫克/千克体重/天的剂量连续口服 EEAP 4 周。研究人员检测了心脏组织中白细胞介素-1β（IL-1β）和核苷酸结合域、富含亮氨酸的家族、含吡啶结构域-3（NLRP3）的 mRNA 表达水平，以及活化 B 细胞的核因子卡巴轻链增强子（NFκB）的浓度和钙水平。此外，还分析了血液学参数（包括血细胞比容、血红蛋白和红细胞）、血液中的天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）和丙二醛（MDA）水平：与纯DOX组相比，EEAP剂量依赖性地降低了IL-1β的mRNA表达（p<0.05），并有降低NLRP3的mRNA表达以及心脏组织中NFκB和钙浓度的趋势。此外，EEAP剂量依赖性地降低了ALP值（p<0.0001），并倾向于改善血液学参数以及血清中的AST和MDA水平：结论：该提取物可预防 DOX 引起的心脏炎症、贫血和肝毒性。结论：穿心莲提取物可预防 DOX 引起的心脏炎症、贫血和肝毒性，但还需要进一步的研究来证实这些发现，包括该提取物对接受 DOX 治疗的癌症大鼠的疗效。

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Molecular and Cellular Biomedical Sciences

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